Detailed information of TA system TA185503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3832022..3832800
• Replicon: chromosome
• Accession: NZ_CP066963
• Organism: Xanthomonas campestris strain 3054

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: JH273_RS16685
• Protein ID: WP_223646366.1
• Coordinates: 3832022..3832513 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: G0CD67
• Locus tag: JH273_RS16690
• Protein ID: WP_014508712.1
• Coordinates: 3832510..3832800 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH273_RS16670 (JH273_16645)	3828350..3829225	+	876	WP_070689999.1	30S ribosomal protein S6--L-glutamate ligase	-
JH273_RS16675 (JH273_16650)	3829875..3830552	+	678	WP_003490678.1	response regulator transcription factor	-
JH273_RS16680 (JH273_16655)	3830545..3831879	+	1335	WP_274507688.1	HAMP domain-containing sensor histidine kinase	-
JH273_RS16685 (JH273_16660)	3832022..3832513	-	492	WP_223646366.1	GNAT family N-acetyltransferase	Toxin
JH273_RS16690 (JH273_16665)	3832510..3832800	-	291	WP_014508712.1	DUF1778 domain-containing protein	Antitoxin
JH273_RS16695 (JH273_16670)	3832876..3833277	-	402	WP_274507689.1	SymE family type I addiction module toxin	-
JH273_RS16700 (JH273_16675)	3833876..3834856	+	981	WP_064510573.1	IS5-like element IS1646 family transposase	-
JH273_RS16705 (JH273_16680)	3834972..3835595	-	624	WP_274507690.1	dephospho-CoA kinase	-
JH273_RS16710 (JH273_16685)	3835609..3836472	-	864	WP_040940797.1	A24 family peptidase	-
JH273_RS16715 (JH273_16690)	3836479..3837741	-	1263	WP_274507691.1	type II secretion system F family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3833876..3834856	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17610.25 Da
• Isoelectric Point: 8.1192

>T185503 WP_223646366.1 NZ_CP066963:c3832513-3832022 [Xanthomonas campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLAVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185503 NZ_CP088270:c147401-146958 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10648.20 Da
• Isoelectric Point: 10.5480

>AT185503 WP_014508712.1 NZ_CP066963:c3832800-3832510 [Xanthomonas campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLIAASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185503 NZ_CP088270:c147628-147398 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A3E1LB51