Detailed information of TA system TA185501

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3787557..3788335
• Replicon: chromosome
• Accession: NZ_CP066961
• Organism: Xanthomonas campestris pv. incanae strain 5057

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: JH262_RS16805
• Protein ID: WP_274392618.1
• Coordinates: 3787844..3788335 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH262_RS16800
• Protein ID: WP_011038219.1
• Coordinates: 3787557..3787847 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH262_RS16775 (JH262_16710)	3783020..3783430	-	411	WP_228424134.1	pilin	-
JH262_RS16780 (JH262_16715)	3783782..3785041	+	1260	WP_228424132.1	type II secretion system F family protein	-
JH262_RS16785 (JH262_16720)	3785048..3785911	+	864	WP_223646361.1	A24 family peptidase	-
JH262_RS16790 (JH262_16725)	3785925..3786548	+	624	WP_274390763.1	dephospho-CoA kinase	-
JH262_RS16795 (JH262_16730)	3787080..3787481	+	402	WP_274392617.1	SymE family type I addiction module toxin	-
JH262_RS16800 (JH262_16735)	3787557..3787847	+	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH262_RS16805 (JH262_16740)	3787844..3788335	+	492	WP_274392618.1	GNAT family N-acetyltransferase	Toxin
JH262_RS16810 (JH262_16745)	3788478..3789812	-	1335	WP_019237347.1	HAMP domain-containing sensor histidine kinase	-
JH262_RS16815 (JH262_16750)	3789805..3790482	-	678	WP_003490678.1	response regulator transcription factor	-
JH262_RS16820 (JH262_16755)	3791133..3792008	-	876	WP_076054142.1	30S ribosomal protein S6--L-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17622.25 Da
• Isoelectric Point: 8.1192

>T185501 WP_274392618.1 NZ_CP066961:3787844-3788335 [Xanthomonas campestris pv. incanae]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALVQDAGKRILHAADTIGIRGLLVHALSSDAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185501 NZ_CP088270:52323-52805 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185501 WP_011038219.1 NZ_CP066961:3787557-3787847 [Xanthomonas campestris pv. incanae]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185501 NZ_CP088270:52069-52335 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: Q8P668