Detailed information of TA system TA185499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3660362..3661140
• Replicon: chromosome
• Accession: NZ_CP066959
• Organism: Xanthomonas campestris pv. incanae strain 4053

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: JH260_RS16085
• Protein ID: WP_274392618.1
• Coordinates: 3660649..3661140 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH260_RS16080
• Protein ID: WP_011038219.1
• Coordinates: 3660362..3660652 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH260_RS16055 (JH260_16025)	3655809..3656234	-	426	WP_031423879.1	pilin	-
JH260_RS16060 (JH260_16030)	3656587..3657846	+	1260	WP_274394336.1	type II secretion system F family protein	-
JH260_RS16065 (JH260_16035)	3657853..3658716	+	864	WP_040940797.1	A24 family peptidase	-
JH260_RS16070 (JH260_16040)	3658730..3659353	+	624	WP_228424129.1	dephospho-CoA kinase	-
JH260_RS16075 (JH260_16045)	3659885..3660286	+	402	WP_274394337.1	SymE family type I addiction module toxin	-
JH260_RS16080 (JH260_16050)	3660362..3660652	+	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH260_RS16085 (JH260_16055)	3660649..3661140	+	492	WP_274392618.1	GNAT family N-acetyltransferase	Toxin
JH260_RS16090 (JH260_16060)	3661283..3662617	-	1335	WP_019237347.1	HAMP domain-containing sensor histidine kinase	-
JH260_RS16095 (JH260_16065)	3662610..3663287	-	678	WP_003490678.1	response regulator transcription factor	-
JH260_RS16100 (JH260_16070)	3663937..3664812	-	876	WP_070689999.1	30S ribosomal protein S6--L-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17622.25 Da
• Isoelectric Point: 8.1192

>T185499 WP_274392618.1 NZ_CP066959:3660649-3661140 [Xanthomonas campestris pv. incanae]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALVQDAGKRILHAADTIGIRGLLVHALSSDAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185499 NZ_CP088269:c4975995-4975078 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCAGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185499 WP_011038219.1 NZ_CP066959:3660362-3660652 [Xanthomonas campestris pv. incanae]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185499 NZ_CP088269:c4976759-4976001 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: Q8P668