Detailed information of TA system TA185497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3853801..3854579
• Replicon: chromosome
• Accession: NZ_CP066958
• Organism: Xanthomonas campestris strain 11-19

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: JH302_RS17115
• Protein ID: WP_274392618.1
• Coordinates: 3854088..3854579 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH302_RS17110
• Protein ID: WP_011038219.1
• Coordinates: 3853801..3854091 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH302_RS17085 (JH302_16945)	3849527..3850789	+	1263	WP_274501635.1	type II secretion system F family protein	-
JH302_RS17090 (JH302_16950)	3850796..3851659	+	864	WP_274501636.1	A24 family peptidase	-
JH302_RS17095 (JH302_16955)	3851673..3852296	+	624	WP_116889149.1	dephospho-CoA kinase	-
JH302_RS17100 (JH302_16960)	3852484..3853470	-	987	WP_274393701.1	IS3 family transposase	-
JH302_RS17105	3853437..3853586	-	150	Protein_3317	transposase	-
JH302_RS17110 (JH302_16965)	3853801..3854091	+	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH302_RS17115 (JH302_16970)	3854088..3854579	+	492	WP_274392618.1	GNAT family N-acetyltransferase	Toxin
JH302_RS17120 (JH302_16975)	3854722..3856056	-	1335	WP_019237347.1	HAMP domain-containing sensor histidine kinase	-
JH302_RS17125 (JH302_16980)	3856049..3856726	-	678	WP_003490678.1	response regulator transcription factor	-
JH302_RS17130 (JH302_16985)	3857376..3858251	-	876	WP_070689999.1	30S ribosomal protein S6--L-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3769152..3990499	221347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17622.25 Da
• Isoelectric Point: 8.1192

>T185497 WP_274392618.1 NZ_CP066958:3854088-3854579 [Xanthomonas campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALVQDAGKRILHAADTIGIRGLLVHALSSDAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185497 NZ_CP088269:c4740264-4739923 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAAATCCGCAGGCGGCGACGCTATGTCTGTCATCCGTGGCAGTCTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCGTTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGACAGACAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185497 WP_011038219.1 NZ_CP066958:3853801-3854091 [Xanthomonas campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185497 NZ_CP088269:c4740626-4740285 [Klebsiella pneumoniae]
ATGAAATCTACTGATTCAGAGAGCGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTGCACTATTTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAACGAAGCAGAATGTC
TTAAGCTGGATGCGGCATTCCCGCATTTAATCTGCCAGATGGAATCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACCCCACTCATCGTTAA

Structures

Antitoxin

• AlphaFold DB: Q8P668