Detailed information of TA system TA185494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3793473..3794251
• Replicon: chromosome
• Accession: NZ_CP066956
• Organism: Xanthomonas campestris pv. incanae strain 8-14

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: JH279_RS16655
• Protein ID: WP_274391841.1
• Coordinates: 3793760..3794251 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: G0CD67
• Locus tag: JH279_RS16650
• Protein ID: WP_014508712.1
• Coordinates: 3793473..3793763 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH279_RS16625 (JH279_16570)	3788960..3789400	-	441	WP_274390762.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
JH279_RS16630 (JH279_16575)	3789701..3790957	+	1257	WP_274391840.1	type II secretion system F family protein	-
JH279_RS16635 (JH279_16580)	3790964..3791827	+	864	WP_057671659.1	A24 family peptidase	-
JH279_RS16640 (JH279_16585)	3791841..3792464	+	624	WP_274390763.1	dephospho-CoA kinase	-
JH279_RS16645 (JH279_16590)	3792996..3793397	+	402	WP_274390764.1	SymE family type I addiction module toxin	-
JH279_RS16650 (JH279_16595)	3793473..3793763	+	291	WP_014508712.1	DUF1778 domain-containing protein	Antitoxin
JH279_RS16655 (JH279_16600)	3793760..3794251	+	492	WP_274391841.1	GNAT family N-acetyltransferase	Toxin
JH279_RS16660 (JH279_16605)	3794394..3795728	-	1335	WP_019237347.1	HAMP domain-containing sensor histidine kinase	-
JH279_RS16665 (JH279_16610)	3795721..3796398	-	678	WP_003490678.1	response regulator transcription factor	-
JH279_RS16670 (JH279_16615)	3797048..3797923	-	876	WP_070689999.1	30S ribosomal protein S6--L-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17578.19 Da
• Isoelectric Point: 8.1192

>T185494 WP_274391841.1 NZ_CP066956:3793760-3794251 [Xanthomonas campestris pv. incanae]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALVQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLAVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185494 NZ_CP088269:c2722382-2721060 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTACTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAGTTTCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10648.20 Da
• Isoelectric Point: 10.5480

>AT185494 WP_014508712.1 NZ_CP066956:3793473-3793763 [Xanthomonas campestris pv. incanae]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLIAASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185494 NZ_CP088269:c2722648-2722382 [Klebsiella pneumoniae]
ATGAATTCCCCCCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGATGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3E1LB51