Detailed information of TA system TA185491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1323568..1324346
• Replicon: chromosome
• Accession: NZ_CP066955
• Organism: Xanthomonas campestris pv. campestris strain 08288

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH309_RS05620
• Protein ID: WP_011038220.1
• Coordinates: 1323568..1324059 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH309_RS05625
• Protein ID: WP_011038219.1
• Coordinates: 1324056..1324346 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH309_RS05605 (JH309_05565)	1319896..1320771	+	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-
JH309_RS05610 (JH309_05570)	1321421..1322098	+	678	WP_003490678.1	response regulator transcription factor	-
JH309_RS05615 (JH309_05575)	1322091..1323425	+	1335	WP_019237347.1	HAMP domain-containing sensor histidine kinase	-
JH309_RS05620 (JH309_05580)	1323568..1324059	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH309_RS05625 (JH309_05585)	1324056..1324346	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH309_RS05630 (JH309_05590)	1324421..1324822	-	402	WP_019237348.1	SymE family type I addiction module toxin	-
JH309_RS05635 (JH309_05595)	1325354..1325977	-	624	WP_011038217.1	dephospho-CoA kinase	-
JH309_RS05640 (JH309_05600)	1325991..1326854	-	864	WP_011038216.1	A24 family peptidase	-
JH309_RS05645 (JH309_05605)	1326861..1328117	-	1257	WP_011038215.1	type II secretion system F family protein	-
JH309_RS05650 (JH309_05610)	1328444..1328884	+	441	WP_011038214.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1119473..1386889	267416

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185491 WP_011038220.1 NZ_CP066955:c1324059-1323568 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185491 NZ_CP088269:356975-357322 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATTCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGAGCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185491 WP_011038219.1 NZ_CP066955:c1324346-1324056 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185491 NZ_CP088269:357322-357621 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668