Detailed information of TA system TA185489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1259381..1260159
• Replicon: chromosome
• Accession: NZ_CP066953
• Organism: Xanthomonas campestris pv. campestris strain 10103

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH282_RS05310
• Protein ID: WP_011038220.1
• Coordinates: 1259381..1259872 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH282_RS05315
• Protein ID: WP_011038219.1
• Coordinates: 1259869..1260159 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH282_RS05295 (JH282_05270)	1255709..1256584	+	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-
JH282_RS05300 (JH282_05275)	1257234..1257911	+	678	WP_003490678.1	response regulator transcription factor	-
JH282_RS05305 (JH282_05280)	1257904..1259238	+	1335	WP_057671653.1	HAMP domain-containing sensor histidine kinase	-
JH282_RS05310 (JH282_05285)	1259381..1259872	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH282_RS05315 (JH282_05290)	1259869..1260159	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH282_RS05320 (JH282_05295)	1260234..1260347	-	114	Protein_1029	SymE family type I addiction module toxin	-
JH282_RS05325 (JH282_05300)	1260399..1261501	+	1103	WP_274376085.1	IS3 family transposase	-
JH282_RS05330 (JH282_05305)	1262024..1262647	-	624	WP_011038217.1	dephospho-CoA kinase	-
JH282_RS05335 (JH282_05310)	1262661..1263524	-	864	WP_057671659.1	A24 family peptidase	-
JH282_RS05340 (JH282_05315)	1263531..1264793	-	1263	WP_057671824.1	type II secretion system F family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185489 WP_011038220.1 NZ_CP066953:c1259872-1259381 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185489 NZ_CP088268:c733230-732880 [Metabacillus litoralis]
TTGATTGTTAAACGAGGCGACGTTTATTTTGCCGATCTTTCCCCAGTTGTAGGTTCAGAGCAAGGTGGCGTACGACCTGT
ATTAATTATTCAAAATGATATTGGGAACCGCTTTAGTCCTACAGTTATTATAGCGGCGATTACGGCCCAAATTCAAAAAG
CAAAATTGCCAACTCATGTTGAGATTGATGCAAAGCGTTACGGGTTTGAAAGGGATTCGGTTATCCTGCTTGAGCAGATT
CGAACGATTGATAAACAAAGGCTTACTGATAAAATTACCCATCTTGATGATGAGATGATGGATAAAGTTGATGAAGCCTT
ACAAATAAGCTTAGGACTTATCGATTTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185489 WP_011038219.1 NZ_CP066953:c1260159-1259869 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185489 NZ_CP088268:c733516-733235 [Metabacillus litoralis]
GTGTCTGAATCCAGCGCAACAACAGAAATCTTAATTCAGTTACCTCAAGCATTAGTATCAGAATTGGATGTATTGGTAAA
ACAAGAGAACGGGAACAGAAATGAGCTTATTTATCAAGCGACAAAAATGTATATTCGTGAGCGTAAAAAGCGTCAAATTC
GTGAATCTATGAGACGTGGCTACATGGAAATGGCAAAAATCAATTTGAATATTGCATCTGAAGCATTTTTGGCAGAATCT
GAGGCTGACCATACCGTTGAACGCTTAGTCAGCGGGGGTTAA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668