Detailed information of TA system TA185487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1239710..1240488
• Replicon: chromosome
• Accession: NZ_CP066949
• Organism: Xanthomonas campestris pv. campestris strain 0659

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH278_RS05225
• Protein ID: WP_011038220.1
• Coordinates: 1239710..1240201 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: JH278_RS05230
• Protein ID: WP_274375532.1
• Coordinates: 1240198..1240488 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH278_RS05210 (JH278_05200)	1236038..1236913	+	876	WP_011038222.1	30S ribosomal protein S6--L-glutamate ligase	-
JH278_RS05215 (JH278_05205)	1237563..1238240	+	678	WP_003490678.1	response regulator transcription factor	-
JH278_RS05220 (JH278_05210)	1238233..1239567	+	1335	WP_227971331.1	HAMP domain-containing sensor histidine kinase	-
JH278_RS05225 (JH278_05215)	1239710..1240201	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH278_RS05230 (JH278_05220)	1240198..1240488	-	291	WP_274375532.1	DUF1778 domain-containing protein	Antitoxin
JH278_RS05235 (JH278_05225)	1240564..1240965	-	402	WP_076037054.1	SymE family type I addiction module toxin	-
JH278_RS05240 (JH278_05230)	1241497..1242120	-	624	WP_040940796.1	dephospho-CoA kinase	-
JH278_RS05245 (JH278_05235)	1242134..1242997	-	864	WP_057671659.1	A24 family peptidase	-
JH278_RS05250 (JH278_05240)	1243004..1244266	-	1263	WP_057671824.1	type II secretion system F family protein	-
JH278_RS05255 (JH278_05245)	1244608..1245036	+	429	WP_057671660.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1160910..1256224	95314
	-	flank	IS/Tn	-	-	1245136..1246473	1337

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185487 WP_011038220.1 NZ_CP066949:c1240201-1239710 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185487 NZ_CP088265:c3600618-3600229 [Leclercia sp. G3L]
ATGGGGATCTATCTTACGCCTGAATTTGAGGCGGAGCGCAAGGATGCACGCATCAGTAATACTATTTTGTGTAAGGCCGC
AAACGCGATCATTTCTGGCCTGCATGGCGATCATTTAGGTAAATACACATATAAAAAGCGGCTTGCCCTGCCAGCCGTGA
GTCCACGAGATGGCGCGCGGGCTATCGTGTTTTTTCATGATGGTGCCAATGTATATTTTTTTGATATGTACCTGAAAAGC
CATCTATCAAAAAAGAAAGGCAAGGAGCTTGAAGATGACGAAATTGATGCTTATTGCAGGATCGCAGAAGATTTTATCGC
GCTGACCCCAGTCCAGATAAAGCGTCTGGTGGATGACAAAGAACTGCTAGAGGTAACTTGTGATGACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10674.22 Da
• Isoelectric Point: 10.5480

>AT185487 WP_274375532.1 NZ_CP066949:c1240488-1240198 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFVLDAARSAAEEVLLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185487 NZ_CP088265:c3600236-3599919 [Leclercia sp. G3L]
ATGACTGAACGACTGAAACGCATTCAGGGCATGGCTCAGCGTTACAACAAATTAGGTGTTGTGAAAGATGAGACGGTGGC
ATCCATCGATGCCGCAGTGGCTGCCCGTAACATCCCGGCGGTACGCGAAATGACCGGCGGCGAGATTAAAGAAGTCCGAG
CGCGCTACAACATGTCTCAGGCGGCCCTGGCAATAACAACCGGTATGTCGGTTGAAAGCGTATCCAAATGGGAGCGCAAT
GAAAGTAAGCCCAACAAAGCGGCCCTACGTATCATCAATATCATTGATGTAAAAGGACCTGAAGTATTCATGGTCTGA

Structures

Toxin

• AlphaFold DB: Q8P667