Detailed information of TA system TA185485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1241223..1242001
• Replicon: chromosome
• Accession: NZ_CP066948
• Organism: Xanthomonas campestris pv. campestris strain 0793

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH266_RS05225
• Protein ID: WP_011038220.1
• Coordinates: 1241223..1241714 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: JH266_RS05230
• Protein ID: WP_274375532.1
• Coordinates: 1241711..1242001 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH266_RS05210 (JH266_05210)	1237551..1238426	+	876	WP_011038222.1	30S ribosomal protein S6--L-glutamate ligase	-
JH266_RS05215 (JH266_05215)	1239076..1239753	+	678	WP_003490678.1	response regulator transcription factor	-
JH266_RS05220 (JH266_05220)	1239746..1241080	+	1335	WP_227971331.1	HAMP domain-containing sensor histidine kinase	-
JH266_RS05225 (JH266_05225)	1241223..1241714	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH266_RS05230 (JH266_05230)	1241711..1242001	-	291	WP_274375532.1	DUF1778 domain-containing protein	Antitoxin
JH266_RS05235 (JH266_05235)	1242077..1242478	-	402	WP_076037054.1	SymE family type I addiction module toxin	-
JH266_RS05240 (JH266_05240)	1243010..1243633	-	624	WP_040940796.1	dephospho-CoA kinase	-
JH266_RS05245 (JH266_05245)	1243647..1244510	-	864	WP_057671659.1	A24 family peptidase	-
JH266_RS05250 (JH266_05250)	1244517..1245779	-	1263	WP_057671824.1	type II secretion system F family protein	-
JH266_RS05255 (JH266_05255)	1246121..1246549	+	429	WP_057671660.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1160911..1256225	95314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185485 WP_011038220.1 NZ_CP066948:c1241714-1241223 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185485 NZ_CP088265:2906537-2906869 [Leclercia sp. G3L]
ATGGATAGAGGGGAAATCTGGCTCGTTTCACTTGATCCTATTGCCGGGCATGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCTGCGTTCAATCATCTCACCCGCCTGCCTGTTGTGGTTCCTGTCACCACTGGCGGCAAGTTTGCCCGTA
CTGCGGGTTTTGCCGTATCCCTGGATGAAGCGGGCTCAAAAACCAGGGGCGTGATTCGCTGCGATCAGCCTCGTACCATA
GATATGGGAGCACGGCATGGCATAAAGTTGGAGCGTGTGCCGGATAGCGTAATGAATGAGGTACTTGCGAAGTTGGGTGC
TTTGTTAACCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10674.22 Da
• Isoelectric Point: 10.5480

>AT185485 WP_274375532.1 NZ_CP066948:c1242001-1241711 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFVLDAARSAAEEVLLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185485 NZ_CP088265:2906279-2906536 [Leclercia sp. G3L]
ATGTACATAACACGTCTGAAAAAAGTGGGCGGCTCTGTCATGCTGGCAGTCCCTCCAGCGATTTTGAAAACGCTCGACCT
GCAACCAGACAGCGAGGTCAGCGTCACTATCGACAACGGCTGCCTGATAGTTGAACCGCAAAAACTCCCGCATTATTCAC
TTGCTGAACTGCTGGCGCAGTGCGATCCGCACGCCGAACTCAGTGAAGAAGATCGAGAATGGGTTGATGCGCCAGCTGCA
GGCAAGGAGATCCTGTAA

Structures

Toxin

• AlphaFold DB: Q8P667