Detailed information of TA system TA185483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1241216..1241994
• Replicon: chromosome
• Accession: NZ_CP066947
• Organism: Xanthomonas campestris pv. campestris strain 0792

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH257_RS05225
• Protein ID: WP_011038220.1
• Coordinates: 1241216..1241707 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: JH257_RS05230
• Protein ID: WP_274375532.1
• Coordinates: 1241704..1241994 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH257_RS05210 (JH257_05210)	1237544..1238419	+	876	WP_011038222.1	30S ribosomal protein S6--L-glutamate ligase	-
JH257_RS05215 (JH257_05215)	1239069..1239746	+	678	WP_003490678.1	response regulator transcription factor	-
JH257_RS05220 (JH257_05220)	1239739..1241073	+	1335	WP_227971331.1	HAMP domain-containing sensor histidine kinase	-
JH257_RS05225 (JH257_05225)	1241216..1241707	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH257_RS05230 (JH257_05230)	1241704..1241994	-	291	WP_274375532.1	DUF1778 domain-containing protein	Antitoxin
JH257_RS05235 (JH257_05235)	1242070..1242471	-	402	WP_076037054.1	SymE family type I addiction module toxin	-
JH257_RS05240 (JH257_05240)	1243003..1243626	-	624	WP_040940796.1	dephospho-CoA kinase	-
JH257_RS05245 (JH257_05245)	1243640..1244503	-	864	WP_057671659.1	A24 family peptidase	-
JH257_RS05250 (JH257_05250)	1244510..1245772	-	1263	WP_057671824.1	type II secretion system F family protein	-
JH257_RS05255 (JH257_05255)	1246114..1246542	+	429	WP_057671660.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1160904..1256218	95314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185483 WP_011038220.1 NZ_CP066947:c1241707-1241216 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185483 NZ_CP088265:2024606-2025019 [Leclercia sp. G3L]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCGTGGCGCGCCCAGTGGATGTCTCTTTTGCTCCACGGCCTGGTTGCGGC
TTGCATTCTGTTGATGCCATGGCCGCTGAGCTACACCCCATTATGGTTAATCTTACTGTCGCTGGTGGTGTTCGACAGCG
TGCGCAGTCAACGACGCATTAATTCCCGTCAGGGCGAAATCAAACTGTTCATGGATTCCCGACTGCACTGGCAGGGCAGC
GAGTGGGAGATTGTCGGCACGCCGTGGATGCTAAATGCCGGCATGATGCTCCGCTTACGCAAAGGCGACGGGAAACGCTA
CCAGCATTTGTGGCTTGCGGCAGACAGCATGGACGAAGGGGAGTGGCGGGATCTGCGCCGGATGCTGTCGCAGCAACCGA
CGCAGGGGCGATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10674.22 Da
• Isoelectric Point: 10.5480

>AT185483 WP_274375532.1 NZ_CP066947:c1241994-1241704 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFVLDAARSAAEEVLLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185483 NZ_CP088265:2024359-2024625 [Leclercia sp. G3L]
ATGGACATTACTAACAAAGCCCGCATTCATTGGGCATGCCGTCGCGGCATGCGCGAACTCGATATCTCCATCATGCCGTT
CTTCGAATACGAATACGACACCCTGAGCGATGAGGACAAACAGCTCTTCATCCGTCTGCTGGAGAACGACGATCCTGATT
TATTTAACTGGTTGATGAATCACGGCGAACCTGCCGACGCCGAACTGCAACGGATGGTGCTTTTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: Q8P667