Detailed information of TA system TA185477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1212575..1213353
• Replicon: chromosome
• Accession: NZ_CP066939
• Organism: Xanthomonas campestris pv. campestris strain 12112

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH286_RS05340
• Protein ID: WP_011038220.1
• Coordinates: 1212575..1213066 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH286_RS05345
• Protein ID: WP_011038219.1
• Coordinates: 1213063..1213353 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH286_RS05325 (JH286_05330)	1208903..1209778	+	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-
JH286_RS05330 (JH286_05335)	1210428..1211105	+	678	WP_003490678.1	response regulator transcription factor	-
JH286_RS05335 (JH286_05340)	1211098..1212432	+	1335	WP_057671653.1	HAMP domain-containing sensor histidine kinase	-
JH286_RS05340 (JH286_05345)	1212575..1213066	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH286_RS05345 (JH286_05350)	1213063..1213353	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH286_RS05350 (JH286_05355)	1213428..1213829	-	402	WP_040940795.1	SymE family type I addiction module toxin	-
JH286_RS05355 (JH286_05360)	1214361..1214984	-	624	WP_011038217.1	dephospho-CoA kinase	-
JH286_RS05360 (JH286_05365)	1214998..1215861	-	864	WP_057671659.1	A24 family peptidase	-
JH286_RS05365 (JH286_05370)	1215868..1217130	-	1263	WP_057671824.1	type II secretion system F family protein	-
JH286_RS05370 (JH286_05375)	1217472..1217900	+	429	WP_057671660.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185477 WP_011038220.1 NZ_CP066939:c1213066-1212575 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185477 NZ_CP088265:202044-202364 [Leclercia sp. G3L]
ATGAAAACTCTACCTGCAACGACTTTGTGGGCGGCTAAACCATGCCCGTCACCAGTGATCGTATGGCAAATGCTTCTTTC
ACGCCTGCTGGATCAGCACTATGGCCTGACACTGAACGACACGCCATTCAGTGATGAAACTGTGATTAAGGAACATATCG
ATGCCGGTATCTCTTTAGCCGATGCCGTTAATTTTCTGGTGGAAAAATACGAACTGGTTCGCATCGATCGTAAGGGGTTT
AGCTGGCAAGAACAGTCGCCTTTTATTACTGCTGTAGACATTCTAAAAGCCAGACGGGCTATGAGGGAAATGAGAATTTA
A

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185477 WP_011038219.1 NZ_CP066939:c1213353-1213063 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185477 NZ_CP088265:201706-202023 [Leclercia sp. G3L]
ATGAGGAACATCACATGGGGGCTGCAGCGGGATATCACGCCACGCCTGGGAGCCCGTCTGGTGCAGGAGGGGAACCGGCT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGTAAATTCAGTGACGTCGAATGCCGTAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAGTCGATGCTAACCACCGATGAACTGAATCCCCGCCATGCCCACTGCGTCACCCTGTACCAC
AATGGTTTCACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACATATACATCACCGTTTATCCTACTCAACGATAA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668