Detailed information of TA system TA185473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1289080..1289858
• Replicon: chromosome
• Accession: NZ_CP066937
• Organism: Xanthomonas campestris pv. campestris strain 13108G

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH313_RS05470
• Protein ID: WP_011038220.1
• Coordinates: 1289080..1289571 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH313_RS05475
• Protein ID: WP_011038219.1
• Coordinates: 1289568..1289858 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH313_RS05455 (JH313_05450)	1285408..1286283	+	876	WP_011038222.1	30S ribosomal protein S6--L-glutamate ligase	-
JH313_RS05460 (JH313_05455)	1286933..1287610	+	678	WP_003490678.1	response regulator transcription factor	-
JH313_RS05465 (JH313_05460)	1287603..1288937	+	1335	WP_019237347.1	HAMP domain-containing sensor histidine kinase	-
JH313_RS05470 (JH313_05465)	1289080..1289571	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH313_RS05475 (JH313_05470)	1289568..1289858	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH313_RS05480 (JH313_05475)	1289933..1290334	-	402	WP_019237348.1	SymE family type I addiction module toxin	-
JH313_RS05485 (JH313_05480)	1290866..1291489	-	624	WP_011038217.1	dephospho-CoA kinase	-
JH313_RS05490 (JH313_05485)	1291503..1292366	-	864	WP_011038216.1	A24 family peptidase	-
JH313_RS05495 (JH313_05490)	1292373..1293629	-	1257	WP_011038215.1	type II secretion system F family protein	-
JH313_RS05500 (JH313_05495)	1293956..1294396	+	441	WP_011038214.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185473 WP_011038220.1 NZ_CP066937:c1289571-1289080 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185473 NZ_CP088252:c1838760-1838476 [Frateuria soli]
ATGACCTATAGCCTGGAGTTCGTCGAGTCCGCGCTCAAGGAATGGCGCAAGCTTTCGCCCGACATCCGCGCGCAACTGAA
GAAAAAGCTGGCCGAGCGGCTGGAGCATCCCCACGTGCCCGCCGCCCGGCTGCATGGCATGCCCGACTGCTTCAAGATCA
AGCTGCGCGCCTCTGGCTACCGGCTGGTGTACCAGGTGCACGACAAGGTGCTGGTGGTGACCGTGGTGGCCGTAGGGAAA
CGGGAGAAGGGGTTGACCTATCTTGCGGCGAAGAAACGCGTGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185473 WP_011038219.1 NZ_CP066937:c1289858-1289568 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185473 NZ_CP088252:c1838995-1838750 [Frateuria soli]
ATGGAAGCCATCCTGGCCGGCGCCAGCATCAGCATCACCGAGCTCAAGCGCAACCCCAGCGCCGTGATCGAGGCGGCCGA
CGGCGAGTCGGTCGCCGTGCTGGTGCACAACAAGCCCAGCGCCTACCTGGTGCCCGCGGAAACCTACAAGCGTCTGCTCG
ATCGTCTGGAAGACCTGGAGCTCGCCGAGCTGGTGCGCCAGCGCAAGGGCGAGCGCCGGGTCAAGGTCAAGCTCGATGAC
CTATAG

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668