Detailed information of TA system TA185471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1234277..1235055
• Replicon: chromosome
• Accession: NZ_CP066936
• Organism: Xanthomonas campestris pv. campestris strain 10090

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH310_RS05240
• Protein ID: WP_011038220.1
• Coordinates: 1234277..1234768 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH310_RS05245
• Protein ID: WP_011038219.1
• Coordinates: 1234765..1235055 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH310_RS05220 (JH310_05200)	1230528..1231403	+	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-
JH310_RS05225 (JH310_05205)	1231626..1232099	+	474	WP_012437597.1	hypothetical protein	-
JH310_RS05230 (JH310_05210)	1232130..1232807	+	678	WP_003490678.1	response regulator transcription factor	-
JH310_RS05235 (JH310_05215)	1232800..1234134	+	1335	WP_012437598.1	HAMP domain-containing sensor histidine kinase	-
JH310_RS05240 (JH310_05220)	1234277..1234768	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH310_RS05245 (JH310_05225)	1234765..1235055	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH310_RS05250 (JH310_05230)	1235130..1235531	-	402	WP_019237348.1	SymE family type I addiction module toxin	-
JH310_RS05255 (JH310_05235)	1236063..1236686	-	624	WP_011038217.1	dephospho-CoA kinase	-
JH310_RS05260 (JH310_05240)	1236700..1237563	-	864	WP_011038216.1	A24 family peptidase	-
JH310_RS05265 (JH310_05245)	1237570..1238826	-	1257	WP_011038215.1	type II secretion system F family protein	-
JH310_RS05270 (JH310_05250)	1239153..1239593	+	441	WP_011038214.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185471 WP_011038220.1 NZ_CP066936:c1234768-1234277 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185471 NZ_CP088247:c4640505-4640251 [Methylorubrum sp. B1-46]
ATGGCTGAGGTCGTCTACAGCAAAGCTGCCGTGCAGGCTTTGATCCGGCTCCCAGCCGACGTCTCCCGGAAGATCCGCCT
CAAGATCGAACAATACGCAGCCGACCCTGCGTCACTTGCCAACAACGTGAAGGCTCTCCGGGGCGAGCCCGGCATCCTAC
GCCTGCGGGTTGGAGATTGGCGTGTTCTCTTCACCGAGGAAGGCGTCGTGATTGCCGTCATCAGGATCGCACCACGGGGC
GACGCGTACGACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185471 WP_011038219.1 NZ_CP066936:c1235055-1234765 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185471 NZ_CP088247:c4640229-4639855 [Methylorubrum sp. B1-46]
ATGAACACGCGGATCATCACCACCCCCGGCGGCGAGCGTCTTGTGCTTATGCCGGAATCGGATTTCGAAGCGTTGGTGGC
AGCGGCCGAAGACAACGCCGACCGTACCGCCGTCGCGGAGTTCCGGCGTAAGATCGCTGCGGGCGAGGAGGAACTGGTTC
CAGCTTCCGTCGTGGAGCGTCTTCTGTCCGGCGAAAACCGTGTCCGTGTCTGGCGTGAACACCGCGGCCTGAGCGGGGCC
GCGCTCGCGAAGCAAGCCGGTCTCGCGCAGGCTTACCTGTCGCAGATCGAGACGGGCGCGCGGGAAGGCACCATCGAAAC
GTATGGCAAGCTTGCAGGGGCTCTCAGGGTTTCCCTGAACGATCTCGCGGGCTGA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668