Detailed information of TA system TA185466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1234312..1235090
• Replicon: chromosome
• Accession: NZ_CP066933
• Organism: Xanthomonas campestris pv. campestris strain 10108

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH304_RS05230
• Protein ID: WP_011038220.1
• Coordinates: 1234312..1234803 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH304_RS05235
• Protein ID: WP_011038219.1
• Coordinates: 1234800..1235090 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH304_RS05210 (JH304_05200)	1230563..1231438	+	876	WP_016945000.1	30S ribosomal protein S6--L-glutamate ligase	-
JH304_RS05215 (JH304_05205)	1231661..1232134	+	474	WP_012437597.1	hypothetical protein	-
JH304_RS05220 (JH304_05210)	1232165..1232842	+	678	WP_003490678.1	response regulator transcription factor	-
JH304_RS05225 (JH304_05215)	1232835..1234169	+	1335	WP_012437598.1	HAMP domain-containing sensor histidine kinase	-
JH304_RS05230 (JH304_05220)	1234312..1234803	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH304_RS05235 (JH304_05225)	1234800..1235090	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH304_RS05240 (JH304_05230)	1235165..1235566	-	402	WP_019237348.1	SymE family type I addiction module toxin	-
JH304_RS05245 (JH304_05235)	1236098..1236721	-	624	WP_011038217.1	dephospho-CoA kinase	-
JH304_RS05250 (JH304_05240)	1236735..1237598	-	864	WP_011038216.1	A24 family peptidase	-
JH304_RS05255 (JH304_05245)	1237605..1238861	-	1257	WP_011038215.1	type II secretion system F family protein	-
JH304_RS05260 (JH304_05250)	1239188..1239628	+	441	WP_011038214.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185466 WP_011038220.1 NZ_CP066933:c1234803-1234312 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185466 NZ_CP088244:c3983793-3983515 [Stenotrophomonas maltophilia]
ATGATCCGGTCCTTCCGTTGCAAGCACACCCGCGCCTTGTTTGAAGGCCACTGCGTGCGCGCCTGGCTGCCGATTCGACG
GGCAGCGGAACGCAAGCTGCAGATGCTTGACTCGGCACAGACGCTGGCGTTCCTGCGCAGTCCGCCCGGCAACCGGTTGG
AGGCACTGGCTGGCGACCGCAAAGGGCAGCACAGCCTGCGAATCAACGATCAGTGGCGGCTGTGTTTCATCTGGACCGAC
AGCGGTCCGCAATCCGTGGAAATCGTTGACTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185466 WP_011038219.1 NZ_CP066933:c1235090-1234800 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185466 NZ_CP088244:c3983504-3983205 [Stenotrophomonas maltophilia]
ATGAAGCCAACCAACAATCGCATGCGGGCCATCCATCCCGGCGAGATCCTGCGAGAGGAGTTCCTTTCCCCGCTGGCTCT
GAGCGTGAATGCACTGGCGAGGGAACTGGATGTTCCGGCCACCCGCCTGCATGCCATCGTCCATGGCGAACGTGCGATCA
CCGCCGACACTGCTGCCCGGCTGGCGCGCCATTTCGGAGGTGATGCCGCATCGTGGCTGGCGCTGCAGGCGACTTACGAC
TTGAAGACGCTGCCCACGCTTGGCGAGATCGAGCGCCGCGTGCAGCCGCGGGCGGCGTAA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668