Detailed information of TA system TA185450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1292597..1293375
• Replicon: chromosome
• Accession: NZ_CP066923
• Organism: Xanthomonas campestris pv. incanae strain CFBP1606

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH295_RS05440
• Protein ID: WP_011038220.1
• Coordinates: 1292597..1293088 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH295_RS05445
• Protein ID: WP_011038219.1
• Coordinates: 1293085..1293375 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH295_RS05425 (JH295_05410)	1288925..1289800	+	876	WP_070689999.1	30S ribosomal protein S6--L-glutamate ligase	-
JH295_RS05430 (JH295_05415)	1290450..1291127	+	678	WP_003490678.1	response regulator transcription factor	-
JH295_RS05435 (JH295_05420)	1291120..1292454	+	1335	WP_070690000.1	HAMP domain-containing sensor histidine kinase	-
JH295_RS05440 (JH295_05425)	1292597..1293088	-	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH295_RS05445 (JH295_05430)	1293085..1293375	-	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH295_RS05450 (JH295_05435)	1293450..1293851	-	402	WP_070690001.1	SymE family type I addiction module toxin	-
JH295_RS05455 (JH295_05440)	1294380..1295003	-	624	WP_070690002.1	dephospho-CoA kinase	-
JH295_RS05460 (JH295_05445)	1295017..1295880	-	864	WP_040940797.1	A24 family peptidase	-
JH295_RS05465 (JH295_05450)	1295887..1297146	-	1260	WP_070690003.1	type II secretion system F family protein	-
JH295_RS05470 (JH295_05455)	1297499..1297918	+	420	WP_070690005.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185450 WP_011038220.1 NZ_CP066923:c1293088-1292597 [Xanthomonas campestris pv. incanae]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185450 NZ_CP088229:31715-32029 [Enterobacter kobei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTGATCCAGTTTCCGCAACACAGAGAGGAGCTTCTTTTTCTGGG
GCGAACCATCGAAAAGAGCCATTGCACGACGCCTGTTGCGCTAAGAAAAATCTTTCCAACGCTAGATAATTTGAAGTATC
TCGATAAGCATTATGTTATTGATATTGCTAACAACAACCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185450 WP_011038219.1 NZ_CP066923:c1293375-1293085 [Xanthomonas campestris pv. incanae]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185450 NZ_CP088229:32026-32445 [Enterobacter kobei]
ATGATGATCGTAGCTGATGCAATGAAAGCAACGCATGCCCTTGTCGCGGCGGTTCCTTTATTGGGTGAACATCCAAACGA
GAAAGATTACCAGGACGCACTTGAGCTGGTTGAGTATTTACTGATGAACGAACCTGGCAGCCCTTTGCTGGATATCGTAT
GTGCAAGAATTCGTCGTTACGAAGCCAATCGACCCGAAATTGTCGCCTTACGCCAGGAGATGGAATCTGTCCCAGTGGGC
ATTGCCGTTTTAAGAACCCTTATGGATCAATACAAACTGACTATTTCGGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCAAGGGTGTTGAACGGTCAACGTCAGCTGACGTTGAACCATATAAAAAAACTGGCTGCTCGCTTTGGGGTATCGC
CTGCGCTGTTCATTGAATGA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668