Detailed information of TA system TA185446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 708611..709389
• Replicon: chromosome
• Accession: NZ_CP066919
• Organism: Xanthomonas campestris pv. campestris strain CFBP6943

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: Q8P667
• Locus tag: JH281_RS03050
• Protein ID: WP_011038220.1
• Coordinates: 708898..709389 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH281_RS03045
• Protein ID: WP_011038219.1
• Coordinates: 708611..708901 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH281_RS03020 (JH281_03005)	703690..704892	+	1203	WP_227971345.1	type II secretion system F family protein	-
JH281_RS03025 (JH281_03010)	704899..705762	+	864	WP_040940797.1	A24 family peptidase	-
JH281_RS03030 (JH281_03015)	705776..706399	+	624	WP_274316878.1	dephospho-CoA kinase	-
JH281_RS03040 (JH281_03025)	708135..708536	+	402	WP_040940795.1	SymE family type I addiction module toxin	-
JH281_RS03045 (JH281_03030)	708611..708901	+	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH281_RS03050 (JH281_03035)	708898..709389	+	492	WP_011038220.1	GNAT family N-acetyltransferase	Toxin
JH281_RS03055 (JH281_03040)	709532..710866	-	1335	WP_057671653.1	HAMP domain-containing sensor histidine kinase	-
JH281_RS03060 (JH281_03045)	710859..711536	-	678	WP_003490678.1	response regulator transcription factor	-
JH281_RS03065 (JH281_03050)	712187..713062	-	876	WP_011038222.1	30S ribosomal protein S6--L-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17638.31 Da
• Isoelectric Point: 8.1192

>T185446 WP_011038220.1 NZ_CP066919:708898-709389 [Xanthomonas campestris pv. campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLVVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185446 NZ_CP088221:c4527231-4527046 [Enterobacter hormaechei]
GTGAAAAGTGCGGATATCATTACTGTTCTGGTTAGCCATGGCTGGAAATGTGTAAGAACGAAGGGAAGCCATCATCAGTT
TCGCCATCCAGTGCAAAAAGGGTTGGTTACCGTCCCACATCCTAAAAAGGACATTAAACCAGGCACTCTCGCACAAATCT
GGCGACAGGCGGGAATTAAACACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185446 WP_011038219.1 NZ_CP066919:708611-708901 [Xanthomonas campestris pv. campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185446 NZ_CP088221:c4527031-4526624 [Enterobacter hormaechei]
ATGTTTTATCCGGCTTACATTCATTCTGATTCCGACGGTAGCGCCAGTGGTTTCTTTCCTGACGTTCCGGGGTGTTTTTT
TGCGGGGGATTCACTGGATGATGCTTTTCAGGATGCACGTGATGCATTAACTGCCCATTTTGAAGCGTTGTTTGAAATGG
ATGAAATGTTGCCACTTCCGGGTATGGTTGAAGCGCATCTGGCAAGTCATCCGGAGGATTTCATTGGCGGACAATGGTTG
CTGGTGGATATCAATATGAAACAGTTTGAAGGCAGAGTAGAGCGCATCAATATTACGATACCTCGCCGTTTGCTGGTGAA
GATTGATTCCTTTGTCAGCGAGCATCCACAATTTACTAACCGAAGTGCTTTTCTTGCAGAGGCAGCACGGCGTGTACTGC
CCCGGTGA

Structures

Toxin

• AlphaFold DB: Q8P667

Antitoxin

• AlphaFold DB: Q8P668