Detailed information of TA system TA185445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 2093..2673
• Replicon: plasmid pXHKPN391-2
• Accession: NZ_CP066917
• Organism: Klebsiella pneumoniae strain XHKPN391

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: JG797_RS28615
• Protein ID: WP_071177730.1
• Coordinates: 2093..2407 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: JG797_RS28620
• Protein ID: WP_000093040.1
• Coordinates: 2395..2673 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG797_RS28595	1..732	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
JG797_RS28600	739..1269	+	531	WP_071177729.1	hypothetical protein	-
JG797_RS28605	1296..1475	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
JG797_RS28610	1501..1929	-	429	WP_001140599.1	hypothetical protein	-
JG797_RS28615	2093..2407	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JG797_RS28620	2395..2673	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
JG797_RS28625	2848..3213	-	366	WP_072354022.1	TonB family protein	-
JG797_RS28630	3210..3581	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
JG797_RS28635	3855..4100	-	246	WP_032440458.1	hypothetical protein	-
JG797_RS28640	4427..6112	+	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
JG797_RS28645	6122..6379	+	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
JG797_RS28650	6464..6613	+	150	WP_032440454.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T185445 WP_071177730.1 NZ_CP066917:c2407-2093 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T185445 NZ_CP088221:c3742165-3741755 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTGGTGTTTGATAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGACAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGGGATCTGCGCCGGCTAATCGTGCAACAGCCGA
CGCAGGAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT185445 WP_000093040.1 NZ_CP066917:c2673-2395 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT185445 NZ_CP088221:c3742412-3742146 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1