Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 5695..6338 Replicon plasmid pXHKPN391-1
Accession NZ_CP066916
Organism Klebsiella pneumoniae strain XHKPN391

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag JG797_RS28010 Protein ID WP_001044770.1
Coordinates 5695..6111 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag JG797_RS28015 Protein ID WP_001261282.1
Coordinates 6108..6338 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG797_RS27990 (1798) 1798..2070 - 273 Protein_2 transposase -
JG797_RS28000 (3052) 3052..4074 - 1023 WP_000361404.1 helicase UvrD -
JG797_RS28005 (4059) 4059..5621 - 1563 WP_004206609.1 AAA family ATPase -
JG797_RS28010 (5695) 5695..6111 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
JG797_RS28015 (6108) 6108..6338 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JG797_RS28020 (6295) 6295..6756 + 462 WP_014343465.1 hypothetical protein -
JG797_RS28025 (6917) 6917..7861 + 945 WP_011977810.1 hypothetical protein -
JG797_RS28030 (7898) 7898..8290 + 393 WP_011977811.1 hypothetical protein -
JG797_RS28035 (8348) 8348..8869 + 522 WP_013214008.1 hypothetical protein -
JG797_RS28040 (8915) 8915..9118 + 204 WP_011977813.1 hypothetical protein -
JG797_RS28045 (9148) 9148..10152 + 1005 WP_011977814.1 hypothetical protein -
JG797_RS28050 (10336) 10336..11115 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-65 / fosA3 / blaKPC-2 - 1..87509 87509


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T185440 WP_001044770.1 NZ_CP066916:c6111-5695 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T185440 NZ_CP088221:c944838-944473 [Enterobacter hormaechei]
ATGCAAATTTCAACTGCACCGACCACGGTGCCTGTTTCGTCACGCCTGTCGCCTATCGAGGTCTGGCAGATACTACTCAA
ACATCTTCTGGCACAACACTATGGTCTAACCCTTAATGACACTCCGTTCAGCGATGAAAGCACTATTCGTGAACATATCA
ATGCTGGTATCTCACTCAGCAACGCAGTGAATTTTATCGTCGAAAAATATGAGCTGGTACGTACTGACCGTAGTGGATTT
ACGATTATGGAGCAGTCTCCCTTCATCAGCAGTATCGACATTCTTCGTGCCCGCCGGGCCACTGGATTGATGAAACGTCA
GGGTTATAAATCCATCAGCAATATCACTACTGGTCACCTTCTCTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT185440 WP_001261282.1 NZ_CP066916:c6338-6108 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT185440 NZ_CP088221:c945212-944874 [Enterobacter hormaechei]
ATGACTAAAGAACCTGTTCAGGAACACGTTTGGGGTTTACACAGTCAAACCGTTCCATCATTCAGCGCCCGGTTAGTTCA
GGAAGGTCGACGGCTGCATTTTCTGGCCGACCGTGCCACGCTTCTCGGCACATTCAGCCCGGAACAATCCACAGCGCTGG
ATGCGATCTTCCCGGCATTAACCCAGGATATGGAAACCGCTCTGCTTAGCGGCAAACTCGACCCACGTGAGGCTAAACAA
TACATCCATACCCGCAACGGTTTCGCCTGCGAGTCAGATACGTTGGGGAGTTGCGGTTACGTTTACATCGTAATTTATCC
TCAATCAGAAACTAGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References