Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5555322..5555947 Replicon chromosome
Accession NZ_CP066915
Organism Klebsiella pneumoniae strain XHKPN391

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JG797_RS27615 Protein ID WP_002882817.1
Coordinates 5555322..5555705 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JG797_RS27620 Protein ID WP_004150355.1
Coordinates 5555705..5555947 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG797_RS27600 (5552688) 5552688..5553590 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JG797_RS27605 (5553587) 5553587..5554222 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JG797_RS27610 (5554219) 5554219..5555148 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JG797_RS27615 (5555322) 5555322..5555705 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JG797_RS27620 (5555705) 5555705..5555947 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
JG797_RS27625 (5556152) 5556152..5557069 + 918 WP_002882812.1 alpha/beta hydrolase -
JG797_RS27630 (5557083) 5557083..5558024 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JG797_RS27635 (5558069) 5558069..5558506 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
JG797_RS27640 (5558503) 5558503..5559363 - 861 WP_002882807.1 virulence factor BrkB family protein -
JG797_RS27645 (5559357) 5559357..5559956 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T185439 WP_002882817.1 NZ_CP066915:c5555705-5555322 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T185439 NZ_CP088221:c320208-319906 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT185439 WP_004150355.1 NZ_CP066915:c5555947-5555705 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT185439 NZ_CP088221:c319899-319612 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCTGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References