Detailed information of TA system TA185430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2745497..2747085
• Replicon: chromosome
• Accession: NZ_CP066915
• Organism: Klebsiella pneumoniae strain XHKPN391

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0H3GN97
• Locus tag: JG797_RS13500
• Protein ID: WP_004199851.1
• Coordinates: 2745763..2747085 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: JG797_RS13495
• Protein ID: WP_002904790.1
• Coordinates: 2745497..2745763 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG797_RS13470 (2740647)	2740647..2741570	+	924	WP_004199849.1	LysR family hpxDE operon transcriptional regulator HpxR	-
JG797_RS13475 (2741551)	2741551..2742705	-	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
JG797_RS13480 (2743051)	2743051..2744430	+	1380	WP_004199850.1	nucleobase:cation symporter-2 family protein	-
JG797_RS13485 (2744408)	2744408..2744908	+	501	WP_002904787.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
JG797_RS13490 (2744905)	2744905..2745231	+	327	WP_002904788.1	hydroxyisourate hydrolase	-
JG797_RS13495 (2745497)	2745497..2745763	+	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JG797_RS13500 (2745763)	2745763..2747085	+	1323	WP_004199851.1	type II toxin-antitoxin system HipA family toxin	Toxin
JG797_RS13505 (2747102)	2747102..2748394	-	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
JG797_RS13510 (2748527)	2748527..2749531	-	1005	WP_004199852.1	fimbrial protein	-
JG797_RS13515 (2749519)	2749519..2752020	-	2502	WP_004199853.1	fimbria/pilus outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49128.44 Da
• Isoelectric Point: 7.2487

>T185430 WP_004199851.1 NZ_CP066915:2745763-2747085 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPLEQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T185430 NZ_CP088203:c1801041-1800775 [Enterococcus durans]
GTGACCTATCATTTAGAATTCACGCCTGAAATTCAGAAACAACTTCGTAAAATGGACAAATATCAAGCTACATTGATTGT
TCGATGGTTGTATCAACATATTGATGGCGTTGATGACCCGAGAAAATTCGGGAAAGGGTTGACAGCCAATCGTTCAGGGC
AATGGCGTTATCGGATTGGCAAATATCGAGTGTTGGTAGAAATAGAAGATAATCAAATGGTTGTCATTGCTATTCAAGTG
GGACACCGAAGAAATGTCTATGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT185430 WP_002904790.1 NZ_CP066915:2745497-2745763 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT185430 NZ_CP088203:c1801268-1801038 [Enterococcus durans]
ATGGCTATGATTACGGTACGTGTGAGTGATGCTGAAAAAGAATGGCTTTCTTATATGGCGGAATTTTATGGAATTACTTT
ATCTGAACTATTAAAAACCTATTCGATGGAACAATTAGAAGATGAATATGATCGACAAACAGCTGAAATTGCCCATAAAC
ATTGGATAGAAAGTGGGAAGCCAACTGTATCCATGGAAGAAATACTGAATGAATTTGGAGGACTAAAGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GN97

Antitoxin

• AlphaFold DB: A0A0H3GXD8