Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 717282..718092 Replicon chromosome
Accession NZ_CP066915
Organism Klebsiella pneumoniae strain XHKPN391

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag JG797_RS03495 Protein ID WP_002887280.1
Coordinates 717559..718092 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JG797_RS03490 Protein ID WP_002887278.1
Coordinates 717282..717548 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG797_RS03470 (713154) 713154..713498 - 345 WP_002887266.1 cation efflux system protein CusF -
JG797_RS03475 (713516) 713516..714901 - 1386 WP_002887267.1 efflux transporter outer membrane subunit -
JG797_RS03480 (715073) 715073..715756 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
JG797_RS03485 (715746) 715746..717179 + 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
JG797_RS03490 (717282) 717282..717548 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
JG797_RS03495 (717559) 717559..718092 + 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
JG797_RS03500 (718140) 718140..719261 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T185428 WP_002887280.1 NZ_CP066915:717559-718092 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T185428 NZ_CP088202:c28722-27859 [Enterococcus faecalis]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTC
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGCG
AAACTTTATGAGAAAGACGTAGTAAAACACGTCACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGCTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTACGAAACAAAAATGTATGTTATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCTATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGCTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGACTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT185428 WP_002887278.1 NZ_CP066915:717282-717548 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT185428 NZ_CP088202:c28996-28724 [Enterococcus faecalis]
ATGGCAGTTACATATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References