Detailed information of TA system TA185425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 399452..400122
• Replicon: chromosome
• Accession: NZ_CP066915
• Organism: Klebsiella pneumoniae strain XHKPN391

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: JG797_RS01890
• Protein ID: WP_004213072.1
• Coordinates: 399679..400122 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: JG797_RS01885
• Protein ID: WP_004213073.1
• Coordinates: 399452..399682 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG797_RS01860 (395675)	395675..396574	-	900	WP_004225022.1	nucleotide-binding protein	-
JG797_RS01865 (396564)	396564..396854	-	291	WP_004213078.1	hypothetical protein	-
JG797_RS01870 (397206)	397206..397412	+	207	WP_004213077.1	hypothetical protein	-
JG797_RS01875 (397402)	397402..397695	-	294	WP_004213076.1	hypothetical protein	-
JG797_RS01880 (397711)	397711..398844	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
JG797_RS01885 (399452)	399452..399682	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JG797_RS01890 (399679)	399679..400122	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
JG797_RS01895 (400271)	400271..400522	+	252	WP_186987481.1	hypothetical protein	-
JG797_RS01900 (400545)	400545..400849	-	305	Protein_376	transposase	-
JG797_RS01905 (401266)	401266..401900	+	635	Protein_377	mucoid phenotype regulator RmpA2	-
JG797_RS01910 (402418)	402418..402821	-	404	Protein_378	GAF domain-containing protein	-
JG797_RS01915 (402912)	402912..403832	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
JG797_RS01920 (403881)	403881..404372	-	492	WP_011251327.1	DM13 domain-containing protein	-
JG797_RS01925 (404435)	404435..404701	-	267	WP_147021650.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaSHV-12 / blaOXA-1 / aac(6')-Ib-cr	rmpA / iutA / iucD / iucC / iucB / iucA	238617..482556	243939

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T185425 WP_004213072.1 NZ_CP066915:399679-400122 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T185425 NZ_CP088200:2773006-2773149 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT185425 WP_004213073.1 NZ_CP066915:399452-399682 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT185425 NZ_CP088200:c2772908-2772710 [Enterococcus faecalis]
TTTTTAGGTAATTGTGCTATAATGGTAATGAAAAGAGAGCTATGCTGTAACATACCTCTCTGATGTAGAGCCGTTTAAGA
CGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATT
TCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620