Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 329100..329686 Replicon chromosome
Accession NZ_CP066915
Organism Klebsiella pneumoniae strain XHKPN391

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag JG797_RS01530 Protein ID WP_002920800.1
Coordinates 329318..329686 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag JG797_RS01525 Protein ID WP_002920802.1
Coordinates 329100..329321 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG797_RS01505 (325257) 325257..326183 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
JG797_RS01510 (326180) 326180..327457 + 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
JG797_RS01515 (327454) 327454..328221 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
JG797_RS01520 (328223) 328223..328936 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
JG797_RS01525 (329100) 329100..329321 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JG797_RS01530 (329318) 329318..329686 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
JG797_RS01535 (329959) 329959..331275 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
JG797_RS01540 (331382) 331382..332269 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
JG797_RS01545 (332266) 332266..333111 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
JG797_RS01550 (333113) 333113..334183 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element blaSHV-12 / blaOXA-1 / aac(6')-Ib-cr rmpA / iutA / iucD / iucC / iucB / iucA 238617..482556 243939


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T185423 WP_002920800.1 NZ_CP066915:329318-329686 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T185423 NZ_CP088200:2772637-2772777 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT185423 WP_002920802.1 NZ_CP066915:329100-329321 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT185423 NZ_CP088200:c2772635-2772492 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References