Detailed information of TA system TA185421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 6032..6612
• Replicon: plasmid pXHKPN083-3
• Accession: NZ_CP066913
• Organism: Klebsiella pneumoniae strain XHKPN083

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: JG792_RS28770
• Protein ID: WP_071177730.1
• Coordinates: 6032..6346 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: JG792_RS28775
• Protein ID: WP_000093040.1
• Coordinates: 6334..6612 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG792_RS28745	1976..3940	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
JG792_RS28750	3940..4671	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
JG792_RS28755	4678..5208	+	531	WP_071177729.1	hypothetical protein	-
JG792_RS28760	5235..5414	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
JG792_RS28765	5440..5868	-	429	WP_001140599.1	hypothetical protein	-
JG792_RS28770	6032..6346	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JG792_RS28775	6334..6612	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
JG792_RS28780	6787..7152	-	366	WP_072354022.1	TonB family protein	-
JG792_RS28785	7149..7520	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
JG792_RS28790	7794..8039	-	246	WP_032440458.1	hypothetical protein	-
JG792_RS28795	8366..10051	+	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T185421 WP_071177730.1 NZ_CP066913:c6346-6032 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T185421 NZ_CP088200:c2766860-2766519 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGGCAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT185421 WP_000093040.1 NZ_CP066913:c6612-6334 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT185421 NZ_CP088200:c2767090-2766860 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1