Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 270845..271371 Replicon plasmid pXHKPN083-1
Accession NZ_CP066911
Organism Klebsiella pneumoniae strain XHKPN083

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag JG792_RS27810 Protein ID WP_000323025.1
Coordinates 271084..271371 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag JG792_RS27805 Protein ID WP_000534858.1
Coordinates 270845..271084 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG792_RS27780 266843..268183 + 1341 WP_000589001.1 ISNCY family transposase -
JG792_RS27785 268604..268915 + 312 Protein_284 IS110 family transposase -
JG792_RS27795 270166..270582 + 417 Protein_286 cation-efflux pump -
JG792_RS27800 270716..270820 - 105 Protein_287 hypothetical protein -
JG792_RS27805 270845..271084 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
JG792_RS27810 271084..271371 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
JG792_RS27815 271443..271601 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
JG792_RS27820 272204..273178 + 975 WP_004181899.1 hypothetical protein -
JG792_RS27825 273376..273753 - 378 WP_004181901.1 hypothetical protein -
JG792_RS27830 274443..275510 + 1068 WP_004181903.1 hypothetical protein -
JG792_RS27835 275666..276013 + 348 WP_004181904.1 hypothetical protein -
JG792_RS27840 276092..276325 + 234 WP_004181905.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4 / blaSHV-12 / aac(3)-IId / blaTEM-1B / mph(A) iucA / iucB / iucC / iucD / iutA / rmpA 1..302656 302656


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T185413 WP_000323025.1 NZ_CP066911:271084-271371 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T185413 NZ_CP088198:c2616742-2616401 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACTCATGGGCAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT185413 WP_000534858.1 NZ_CP066911:270845-271084 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT185413 NZ_CP088198:c2616972-2616742 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References