Detailed information of TA system TA185410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 45451..46121
• Replicon: plasmid pXHKPN083-1
• Accession: NZ_CP066911
• Organism: Klebsiella pneumoniae strain XHKPN083

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: JG792_RS26630
• Protein ID: WP_004213072.1
• Coordinates: 45451..45894 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: JG792_RS26635
• Protein ID: WP_004213073.1
• Coordinates: 45891..46121 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG792_RS26595	40863..41138	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
JG792_RS26600	41201..41692	+	492	WP_011251327.1	DM13 domain-containing protein	-
JG792_RS26605	41741..42661	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
JG792_RS26610	42752..43155	+	404	Protein_49	GAF domain-containing protein	-
JG792_RS26615	43673..44307	-	635	Protein_50	mucoid phenotype regulator RmpA2	-
JG792_RS26620	44724..45028	+	305	Protein_51	transposase	-
JG792_RS26625	45051..45302	-	252	WP_186987481.1	hypothetical protein	-
JG792_RS26630	45451..45894	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
JG792_RS26635	45891..46121	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JG792_RS26640	46729..47862	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
JG792_RS26645	47878..48171	+	294	WP_004213076.1	hypothetical protein	-
JG792_RS26650	48161..48367	-	207	WP_004213077.1	hypothetical protein	-
JG792_RS26655	48719..49009	+	291	WP_004213078.1	hypothetical protein	-
JG792_RS26660	48999..49898	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4 / blaSHV-12 / aac(3)-IId / blaTEM-1B / mph(A)	iucA / iucB / iucC / iucD / iutA / rmpA	1..302656	302656

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T185410 WP_004213072.1 NZ_CP066911:c45894-45451 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T185410 NZ_CP088198:c2464960-2464859 [Enterococcus faecalis]
TTGTCAATCGAAGCGACATTGGAATTGATGATTAGTTTTGCAACCCTTGTTGCGTTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACAATAAAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT185410 WP_004213073.1 NZ_CP066911:c46121-45891 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT185410 NZ_CP088198:2464736-2464922 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCCTTTATACCAGCGCCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTTT
TTTATTACTGTTTAACCGTTCGGCCTGTCAAGCTTATGAACGGTTATTTTTTATTGTTTTTCGTTGCTTCAAGGATACCG
AAAATCAGTAACGCAACAAGGGTTGCA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620