Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5285237..5285862 Replicon chromosome
Accession NZ_CP066910
Organism Klebsiella pneumoniae strain XHKPN083

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JG792_RS26000 Protein ID WP_002882817.1
Coordinates 5285237..5285620 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JG792_RS26005 Protein ID WP_004150355.1
Coordinates 5285620..5285862 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG792_RS25985 5282603..5283505 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JG792_RS25990 5283502..5284137 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JG792_RS25995 5284134..5285063 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JG792_RS26000 5285237..5285620 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JG792_RS26005 5285620..5285862 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
JG792_RS26010 5286067..5286984 + 918 WP_002882812.1 alpha/beta hydrolase -
JG792_RS26015 5286998..5287939 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JG792_RS26020 5287984..5288421 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
JG792_RS26025 5288418..5289278 - 861 WP_002882807.1 virulence factor BrkB family protein -
JG792_RS26030 5289272..5289871 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T185409 WP_002882817.1 NZ_CP066910:c5285620-5285237 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T185409 NZ_CP088198:c312593-312498 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGCTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT185409 WP_004150355.1 NZ_CP066910:c5285862-5285620 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT185409 NZ_CP088198:312399-312463 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATTGCAGTAGGGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References