Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4088618..4089237 Replicon chromosome
Accession NZ_CP066910
Organism Klebsiella pneumoniae strain XHKPN083

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag JG792_RS20250 Protein ID WP_002892050.1
Coordinates 4089019..4089237 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag JG792_RS20245 Protein ID WP_002892066.1
Coordinates 4088618..4088992 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG792_RS20235 4083770..4084963 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JG792_RS20240 4084986..4088132 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
JG792_RS20245 4088618..4088992 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
JG792_RS20250 4089019..4089237 + 219 WP_002892050.1 HHA domain-containing protein Toxin
JG792_RS20255 4089396..4089962 + 567 WP_002892030.1 maltose O-acetyltransferase -
JG792_RS20260 4089934..4090074 - 141 WP_004147370.1 hypothetical protein -
JG792_RS20265 4090095..4090565 + 471 WP_002892026.1 YlaC family protein -
JG792_RS20270 4090540..4091991 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
JG792_RS20275 4092092..4092790 + 699 WP_002892021.1 GNAT family protein -
JG792_RS20280 4092787..4092927 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
JG792_RS20285 4092927..4093190 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T185405 WP_002892050.1 NZ_CP066910:4089019-4089237 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T185405 NZ_CP088191:192447-192713 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT185405 WP_002892066.1 NZ_CP066910:4088618-4088992 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT185405 NZ_CP088191:192184-192447 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References