Detailed information of TA system TA185391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 3895..4475
• Replicon: plasmid pXHKP502-4
• Accession: NZ_CP066908
• Organism: Klebsiella pneumoniae strain XHKP502

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: JG791_RS29420
• Protein ID: WP_071177730.1
• Coordinates: 3895..4209 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: JG791_RS29425
• Protein ID: WP_000093040.1
• Coordinates: 4197..4475 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG791_RS29395	1..1803	+	1803	WP_255074406.1	TraM recognition domain-containing protein	-
JG791_RS29400	1803..2534	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
JG791_RS29405	2541..3071	+	531	WP_071177729.1	hypothetical protein	-
JG791_RS29410	3098..3277	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
JG791_RS29415	3303..3731	-	429	WP_001140599.1	hypothetical protein	-
JG791_RS29420	3895..4209	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JG791_RS29425	4197..4475	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
JG791_RS29430	4650..5015	-	366	WP_072354022.1	TonB family protein	-
JG791_RS29435	5012..5383	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
JG791_RS29440	5657..5902	-	246	WP_032440458.1	hypothetical protein	-
JG791_RS29445	6547..8034	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11971	11971

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T185391 WP_071177730.1 NZ_CP066908:c4209-3895 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T185391 NZ_CP088157:c2165710-2165348 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATACAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT185391 WP_000093040.1 NZ_CP066908:c4475-4197 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT185391 NZ_CP088157:c2165877-2165707 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1