Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 5323..5966 Replicon plasmid pXHKP502-2
Accession NZ_CP066906
Organism Klebsiella pneumoniae strain XHKP502

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag JG791_RS28375 Protein ID WP_001044770.1
Coordinates 5550..5966 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag JG791_RS28370 Protein ID WP_001261282.1
Coordinates 5323..5553 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG791_RS28335 (546) 546..1325 - 780 WP_013214009.1 site-specific integrase -
JG791_RS28340 (1509) 1509..2513 - 1005 WP_011977814.1 hypothetical protein -
JG791_RS28345 (2543) 2543..2746 - 204 WP_011977813.1 hypothetical protein -
JG791_RS28350 (2792) 2792..3313 - 522 WP_013214008.1 hypothetical protein -
JG791_RS28355 (3371) 3371..3763 - 393 WP_011977811.1 hypothetical protein -
JG791_RS28360 (3800) 3800..4744 - 945 WP_011977810.1 hypothetical protein -
JG791_RS28365 (4905) 4905..5366 - 462 WP_014343465.1 hypothetical protein -
JG791_RS28370 (5323) 5323..5553 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JG791_RS28375 (5550) 5550..5966 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
JG791_RS28380 (6040) 6040..7602 + 1563 WP_004206609.1 AAA family ATPase -
JG791_RS28385 (7587) 7587..8609 + 1023 WP_000361404.1 helicase UvrD -
JG791_RS28390 (8865) 8865..9562 + 698 WP_223174630.1 IS1 family transposase -
JG791_RS28395 (9591) 9591..9863 + 273 Protein_14 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 / blaSHV-12 / blaCTX-M-65 - 1..85781 85781


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T185386 WP_001044770.1 NZ_CP066906:5550-5966 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T185386 NZ_CP088157:c837771-837583 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT185386 WP_001261282.1 NZ_CP066906:5323-5553 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT185386 NZ_CP088157:c838060-837800 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References