Detailed information of TA system TA185385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 97689..98359
• Replicon: plasmid pXHKP502-1
• Accession: NZ_CP066905
• Organism: Klebsiella pneumoniae strain XHKP502

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: JG791_RS27695
• Protein ID: WP_004213072.1
• Coordinates: 97689..98132 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: JG791_RS27700
• Protein ID: WP_004213073.1
• Coordinates: 98129..98359 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG791_RS27660	93100..93375	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
JG791_RS27665	93438..93929	+	492	WP_011251327.1	DM13 domain-containing protein	-
JG791_RS27670	93978..94898	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
JG791_RS27675	94989..95392	+	404	Protein_110	GAF domain-containing protein	-
JG791_RS27680	95910..96545	-	636	Protein_111	mucoid phenotype regulator RmpA2	-
JG791_RS27685	96962..97266	+	305	Protein_112	transposase	-
JG791_RS27690	97289..97540	-	252	WP_186987481.1	hypothetical protein	-
JG791_RS27695	97689..98132	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
JG791_RS27700	98129..98359	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JG791_RS27705	98967..100100	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
JG791_RS27710	100116..100409	+	294	WP_004213076.1	hypothetical protein	-
JG791_RS27715	100399..100605	-	207	WP_004213077.1	hypothetical protein	-
JG791_RS27720	101577..102398	+	822	WP_004213562.1	hypothetical protein	-
JG791_RS27725	102395..103174	+	780	WP_004213560.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..221472	221472

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T185385 WP_004213072.1 NZ_CP066905:c98132-97689 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T185385 NZ_CP088156:c7976192-7975899 [Bradyrhizobium sp. A19]
ATGCTCGAGGTCCGGCAGACCAGCGAGTTCAAGGAATGGCTTCTTGGTCTGCGAGATGCGAGAGCAAAGGCACGTATTGC
GGCTCGCATTGCCCGTGCTGAAGGTGGCAACCTCGGTGACAGCAAGCCGGTTGGTGGGGGCGTCTCCCAAATGCTGATCG
ATTACGGCCCCGGCTATCGGGTCTATTTGCGCCAGAAAGGCAACGTCCTGGTGATCCTGCTTTGCGGTGGTGACAAGAGC
ATCCAGCGCTCCGATATCCGGCGCGCTCAAGAATTGGCCGAGAGAGAGGGGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT185385 WP_004213073.1 NZ_CP066905:c98359-98129 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT185385 NZ_CP088156:c7975895-7975566 [Bradyrhizobium sp. A19]
ATGGCAACGACGCGCTTCGATGCCGCGGAATATCTTGCAGAGCCGGAGGCCCAAGCCGAGTTTCTGGCGGCGGCGCTTGA
GGACGGCACCGCTGATGAAATCCGCGCCGCGATCAACACAATTGCCCGAGCGCGCGGCATGACGGAAGTCGCCAAGGCAA
CCGGCATCCGTCGCGAACAGCTTTATCGGGCACTTGGTGAAGACGGGAATCCGGAGTTCGCCACCATCCTCAAGGTGATC
CGCGCTCTCGGCGTGAAGCTCACCTCAGCGCCGCCCAAGGCTGCTCCGAAGCGGAAGTCTGCCAAGAAAGCAGGCCGCCG
GGCCGCGTAG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620