Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 51562..52205 Replicon plasmid pXHKP75-2
Accession NZ_CP066897
Organism Klebsiella pneumoniae strain XHKP75

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag JG785_RS28485 Protein ID WP_001044770.1
Coordinates 51562..51978 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag JG785_RS28490 Protein ID WP_001261282.1
Coordinates 51975..52205 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG785_RS28465 (47665) 47665..47937 - 273 Protein_55 transposase -
JG785_RS28475 (48919) 48919..49941 - 1023 WP_000361404.1 helicase UvrD -
JG785_RS28480 (49926) 49926..51488 - 1563 WP_004206609.1 AAA family ATPase -
JG785_RS28485 (51562) 51562..51978 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
JG785_RS28490 (51975) 51975..52205 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JG785_RS28495 (52162) 52162..52623 + 462 WP_014343465.1 hypothetical protein -
JG785_RS28500 (52784) 52784..53728 + 945 WP_011977810.1 hypothetical protein -
JG785_RS28505 (53765) 53765..54157 + 393 WP_011977811.1 hypothetical protein -
JG785_RS28510 (54215) 54215..54736 + 522 WP_013214008.1 hypothetical protein -
JG785_RS28515 (54782) 54782..54985 + 204 WP_011977813.1 hypothetical protein -
JG785_RS28520 (55015) 55015..56019 + 1005 WP_011977814.1 hypothetical protein -
JG785_RS28525 (56203) 56203..56982 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB - 1..163729 163729


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T185333 WP_001044770.1 NZ_CP066897:c51978-51562 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T185333 NZ_CP088138:3515598-3515816 [Salmonella enterica subsp. enterica serovar Hissar]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT185333 WP_001261282.1 NZ_CP066897:c52205-51975 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT185333 NZ_CP088138:3515196-3515570 [Salmonella enterica subsp. enterica serovar Hissar]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGACTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References