Detailed information of TA system TA185332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 195875..196611
• Replicon: plasmid pXHKP75-1
• Accession: NZ_CP066896
• Organism: Klebsiella pneumoniae strain XHKP75

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: JG785_RS28140
• Protein ID: WP_003026803.1
• Coordinates: 196129..196611 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: JG785_RS28135
• Protein ID: WP_003026799.1
• Coordinates: 195875..196141 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG785_RS28090	191937..192299	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
JG785_RS28095	192349..192699	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
JG785_RS28100	193057..193326	+	270	WP_004152102.1	hypothetical protein	-
JG785_RS28105	193314..193889	+	576	WP_004152103.1	hypothetical protein	-
JG785_RS28110	193920..194414	+	495	WP_004152104.1	DNA-binding protein	-
JG785_RS28115	194458..194826	+	369	WP_004152105.1	hypothetical protein	-
JG785_RS28120	194860..195063	+	204	WP_004152106.1	HHA domain-containing protein	-
JG785_RS28125	195112..195369	+	258	WP_004152107.1	hypothetical protein	-
JG785_RS28130	195445..195699	+	255	WP_004152108.1	hypothetical protein	-
JG785_RS28135	195875..196141	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
JG785_RS28140	196129..196611	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
JG785_RS28145	196823..198169	+	1347	WP_020314316.1	ISNCY family transposase	-
JG785_RS28150	198330..198461	+	132	WP_004218042.1	hypothetical protein	-
JG785_RS28155	198670..199835	-	1166	Protein_220	IS3 family transposase	-
JG785_RS28160	200012..200974	-	963	WP_004152113.1	zinc metalloprotease HtpX	-
JG785_RS28165	200961..201488	-	528	WP_255034285.1	phosphate-starvation-inducible PsiE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / dfrA12 / aadA2 / qacE / sul1 / mph(A)	-	1..205681	205681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T185332 WP_003026803.1 NZ_CP066896:196129-196611 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T185332 NZ_CP088138:2460263-2460547 [Salmonella enterica subsp. enterica serovar Hissar]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT185332 WP_003026799.1 NZ_CP066896:195875-196141 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT185332 NZ_CP088138:2460025-2460273 [Salmonella enterica subsp. enterica serovar Hissar]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66