Detailed information of TA system TA185331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 119441..120084
• Replicon: plasmid pXHKP75-1
• Accession: NZ_CP066896
• Organism: Klebsiella pneumoniae strain XHKP75

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: JG785_RS27740
• Protein ID: WP_014386165.1
• Coordinates: 119668..120084 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: JG785_RS27735
• Protein ID: WP_032408893.1
• Coordinates: 119441..119671 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG785_RS27710	114514..115497	-	984	WP_001471744.1	NAD(P)H-quinone oxidoreductase	-
JG785_RS27715	115516..116664	-	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
JG785_RS27720	116835..117992	-	1158	WP_032408895.1	acetylornithine deacetylase	-
JG785_RS27725	118008..118682	-	675	WP_014386166.1	DUF1028 domain-containing protein	-
JG785_RS27730	118687..119121	-	435	WP_000103648.1	RidA family protein	-
JG785_RS27735	119441..119671	+	231	WP_032408893.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JG785_RS27740	119668..120084	+	417	WP_014386165.1	PIN domain-containing protein	Toxin
JG785_RS27745	120407..121375	+	969	WP_074422984.1	IS5-like element IS903B family transposase	-
JG785_RS27750	121555..121929	-	375	WP_032408891.1	hypothetical protein	-
JG785_RS27755	121985..122311	-	327	WP_004152639.1	hypothetical protein	-
JG785_RS27760	122308..123036	-	729	WP_014386162.1	plasmid SOS inhibition protein A	-
JG785_RS27765	123033..123464	-	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / dfrA12 / aadA2 / qacE / sul1 / mph(A)	-	1..205681	205681
	-	flank	IS/Tn	-	-	120407..121375	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T185331 WP_014386165.1 NZ_CP066896:119668-120084 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T185331 NZ_CP088138:2118241-2118344 [Salmonella enterica subsp. enterica serovar Hissar]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT185331 WP_032408893.1 NZ_CP066896:119441-119671 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT185331 NZ_CP088138:c2118346-2118201 [Salmonella enterica subsp. enterica serovar Hissar]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG