Detailed information of TA system TA185330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 95545..96290
• Replicon: plasmid pXHKP75-1
• Accession: NZ_CP066896
• Organism: Klebsiella pneumoniae strain XHKP75

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: JG785_RS27610
• Protein ID: WP_032408901.1
• Coordinates: 95799..96290 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: JG785_RS27605
• Protein ID: WP_014386183.1
• Coordinates: 95545..95811 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG785_RS27555	91097..91510	-	414	WP_013023817.1	helix-turn-helix domain-containing protein	-
JG785_RS27560	91511..91789	-	279	WP_004152721.1	helix-turn-helix transcriptional regulator	-
JG785_RS27565	91779..92099	-	321	WP_014386190.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JG785_RS27570	92180..92404	-	225	WP_014386189.1	hypothetical protein	-
JG785_RS27575	92415..92627	-	213	WP_014386188.1	hypothetical protein	-
JG785_RS27580	92689..93015	-	327	WP_014386187.1	hypothetical protein	-
JG785_RS27585	93652..94002	-	351	WP_014386186.1	hypothetical protein	-
JG785_RS27590	93999..94271	-	273	WP_032408902.1	hypothetical protein	-
JG785_RS27595	94461..94946	+	486	WP_014386185.1	hypothetical protein	-
JG785_RS27600	95190..95348	-	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
JG785_RS27605	95545..95811	+	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
JG785_RS27610	95799..96290	+	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
JG785_RS27615	96731..96982	-	252	WP_032408900.1	aldo/keto reductase	-
JG785_RS27620	97179..98771	-	1593	Protein_113	IS66 family transposase	-
JG785_RS27625	98802..99152	-	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
JG785_RS27630	99149..99589	-	441	WP_014386179.1	transposase	-
JG785_RS27635	99851..100606	-	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / dfrA12 / aadA2 / qacE / sul1 / mph(A)	-	1..205681	205681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T185330 WP_032408901.1 NZ_CP066896:95799-96290 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T185330 NZ_CP088138:c1027395-1026868 [Salmonella enterica subsp. enterica serovar Hissar]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCGGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT185330 WP_014386183.1 NZ_CP066896:95545-95811 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT185330 NZ_CP088138:c1027682-1027392 [Salmonella enterica subsp. enterica serovar Hissar]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACATACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTAAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: A0A331KSM6