Detailed information of TA system TA185311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1647..2121
• Replicon: plasmid pXHKP53-3
• Accession: NZ_CP066894
• Organism: Klebsiella pneumoniae strain XHKP53

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: JG783_RS27770
• Protein ID: WP_004197762.1
• Coordinates: 1855..2121 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: JG783_RS27765
• Protein ID: WP_000879771.1
• Coordinates: 1647..1871 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG783_RS27755	1..177	+	177	WP_255087394.1	hypothetical protein	-
JG783_RS27760	777..987	+	211	Protein_1	MbeD family mobilization/exclusion protein	-
JG783_RS27765	1647..1871	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
JG783_RS27770	1855..2121	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JG783_RS27775	2190..2564	-	375	WP_228733401.1	hypothetical protein	-
JG783_RS27780	2801..3148	-	348	WP_255087391.1	hypothetical protein	-
JG783_RS27785	3174..3500	-	327	WP_050516389.1	hypothetical protein	-
JG783_RS27790	4651..4827	+	177	WP_004197757.1	hypothetical protein	-
JG783_RS27795	4817..5161	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
JG783_RS27800	5151..5390	+	240	WP_255087393.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5567	5567

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T185311 WP_004197762.1 NZ_CP066894:1855-2121 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T185311 NZ_CP088136:357379-357654 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT185311 WP_000879771.1 NZ_CP066894:1647-1871 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT185311 NZ_CP088136:357116-357376 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5