Detailed information of TA system TA185284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 4057..4637
• Replicon: plasmid pXHKP6-2
• Accession: NZ_CP066889
• Organism: Klebsiella pneumoniae strain XHKP6

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: JG758_RS28075
• Protein ID: WP_071177730.1
• Coordinates: 4057..4371 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: JG758_RS28080
• Protein ID: WP_000093040.1
• Coordinates: 4359..4637 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG758_RS28050	1..1965	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
JG758_RS28055	1965..2696	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
JG758_RS28060	2703..3233	+	531	WP_071177729.1	hypothetical protein	-
JG758_RS28065	3260..3439	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
JG758_RS28070	3465..3893	-	429	WP_001140599.1	hypothetical protein	-
JG758_RS28075	4057..4371	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JG758_RS28080	4359..4637	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
JG758_RS28085	4812..5177	-	366	WP_072354022.1	TonB family protein	-
JG758_RS28090	5174..5545	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
JG758_RS28095	5819..6064	-	246	WP_032440458.1	hypothetical protein	-
JG758_RS28100	6391..8076	+	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
JG758_RS28105	8086..8343	+	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
JG758_RS28110	8428..8577	+	150	WP_032440454.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10098	10098

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T185284 WP_071177730.1 NZ_CP066889:c4371-4057 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T185284 NZ_CP088131:c2534314-2534138 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT185284 WP_000093040.1 NZ_CP066889:c4637-4359 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT185284 NZ_CP088131:c2534092-2533676 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1