Detailed information of TA system TA185277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 51593..52236
• Replicon: plasmid pXHKP6-1
• Accession: NZ_CP066888
• Organism: Klebsiella pneumoniae strain XHKP6

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: JG758_RS27145
• Protein ID: WP_001044770.1
• Coordinates: 51593..52009 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: JG758_RS27150
• Protein ID: WP_001261282.1
• Coordinates: 52006..52236 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG758_RS27125 (47696)	47696..47968	-	273	Protein_53	transposase	-
JG758_RS27135 (48950)	48950..49972	-	1023	WP_000361404.1	helicase UvrD	-
JG758_RS27140 (49957)	49957..51519	-	1563	WP_004206609.1	AAA family ATPase	-
JG758_RS27145 (51593)	51593..52009	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
JG758_RS27150 (52006)	52006..52236	-	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JG758_RS27155 (52193)	52193..52654	+	462	WP_014343465.1	hypothetical protein	-
JG758_RS27160 (52815)	52815..53759	+	945	WP_011977810.1	hypothetical protein	-
JG758_RS27165 (53796)	53796..54188	+	393	WP_011977811.1	hypothetical protein	-
JG758_RS27170 (54246)	54246..54767	+	522	WP_013214008.1	hypothetical protein	-
JG758_RS27175 (54813)	54813..55016	+	204	WP_011977813.1	hypothetical protein	-
JG758_RS27180 (55046)	55046..56050	+	1005	WP_011977814.1	hypothetical protein	-
JG758_RS27185 (56234)	56234..57013	+	780	WP_013214009.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-12 / blaKPC-2 / catA2 / blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB	-	1..180300	180300

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T185277 WP_001044770.1 NZ_CP066888:c52009-51593 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T185277 NZ_CP088131:c615658-615194 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT185277 WP_001261282.1 NZ_CP066888:c52236-52006 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT185277 NZ_CP088131:c615993-615658 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3