Detailed information of TA system TA185251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 2637814..2638766
• Replicon: chromosome
• Accession: NZ_CP066853
• Organism: Rhodococcus biphenylivorans strain B403

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: H0JT30
• Locus tag: JGU70_RS12225
• Protein ID: WP_006552790.1
• Coordinates: 2637814..2638383 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: JGU70_RS12230
• Protein ID: WP_200358071.1
• Coordinates: 2638380..2638766 (-)
• Length: 129 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGU70_RS12225	2637814..2638383	-	570	WP_006552790.1	zeta toxin family protein	Toxin
JGU70_RS12230	2638380..2638766	-	387	WP_200358071.1	hypothetical protein	Antitoxin
JGU70_RS12235	2638973..2640043	+	1071	WP_006552788.1	class I SAM-dependent methyltransferase	-
JGU70_RS12240	2640113..2640478	+	366	WP_039585980.1	HNH endonuclease	-
JGU70_RS12245	2640495..2640995	-	501	WP_200358073.1	hypothetical protein	-
JGU70_RS12250	2642109..2643041	-	933	WP_200358075.1	TAXI family TRAP transporter solute-binding subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-136)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (7-79)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 20552.71 Da
• Isoelectric Point: 10.6895

>T185251 WP_006552790.1 NZ_CP066853:c2638383-2637814 [Rhodococcus biphenylivorans]
MRRLDLIVGPNGSGKSTFVELSLAPLLPRSVFVNADVIARQRWPDRAEEMSYEAAQLAAAARDRLLRQGRSFIAETVFSH
PSKLELIDRAHEVGFTVVLHVMLVPEAMSVLRVQHRVASGGHAVPEDKIRARYRRLWPLVTEAIGRADQASVYDTASGTP
VVVARFAGGVPVGAVRWPGWAPAALTGLG

Nucleotide

• Length: 570 bp

>T185251 NZ_CP088123:72371-72787 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 129 a.a.
• Molecular weight: 13981.44 Da
• Isoelectric Point: 4.5925

>AT185251 WP_200358071.1 NZ_CP066853:c2638766-2638380 [Rhodococcus biphenylivorans]
MTSSTDKVTRFDAELVDSAIAEGGRQNRTGRQQLEYWARIGRAMTAHETASLHRVHEALAGTRELSELTAAEGRLFDAEI
DALLADGLARTDYAEVLAARGVSTVVLDDEGRLVEQRPDGSRRVLDDA

Nucleotide

• Length: 387 bp

>AT185251 NZ_CP088123:72144-72374 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A7T7RLW6