Detailed information of TA system TA185246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3887407..3888221
• Replicon: chromosome
• Accession: NZ_CP066851
• Organism: Salmonella enterica subsp. enterica serovar Heidelberg strain SH-2813-Parental

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A633KU44
• Locus tag: JIG31_RS18790
• Protein ID: WP_000971648.1
• Coordinates: 3887694..3888221 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: M7S6I2
• Locus tag: JIG31_RS18785
• Protein ID: WP_000855694.1
• Coordinates: 3887407..3887697 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JIG31_RS18755 (3883356)	3883356..3884006	-	651	WP_001674874.1	type III secretion system transcriptional activator InvF	-
JIG31_RS18760 (3884463)	3884463..3884906	+	444	WP_000715096.1	SPI-1 type III secretion system invasion lipoprotein InvH	-
JIG31_RS18765 (3885338)	3885338..3885787	+	450	WP_000381611.1	hypothetical protein	-
JIG31_RS18770 (3885772)	3885772..3886119	+	348	WP_001669174.1	DUF1493 family protein	-
JIG31_RS18775 (3886392)	3886392..3886718	-	327	WP_000393301.1	hypothetical protein	-
JIG31_RS18780 (3886959)	3886959..3887137	+	179	Protein_3691	IS3 family transposase	-
JIG31_RS18785 (3887407)	3887407..3887697	+	291	WP_000855694.1	DUF1778 domain-containing protein	Antitoxin
JIG31_RS18790 (3887694)	3887694..3888221	+	528	WP_000971648.1	GNAT family N-acetyltransferase	Toxin
JIG31_RS18795 (3888294)	3888294..3888703	-	410	Protein_3694	IS5/IS1182 family transposase	-
JIG31_RS18800 (3888936)	3888936..3889592	+	657	WP_000420452.1	protein-serine/threonine phosphatase	-
JIG31_RS18805 (3889764)	3889764..3890285	-	522	WP_000858988.1	hypothetical protein	-
JIG31_RS18810 (3890444)	3890444..3893011	+	2568	WP_001005807.1	DNA mismatch repair protein MutS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3888294..3888434	140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19040.90 Da
• Isoelectric Point: 9.3905

>T185246 WP_000971648.1 NZ_CP066851:3887694-3888221 [Salmonella enterica subsp. enterica serovar Heidelberg]
MMFTDWHEAAIGKTHNRMNFDCGDADLNQFLQRHALQNHEKGTTKTYVALDNSDITRIHGFYSVSPASLIYAQVPGAISK
GLGRYDVPVFRLGRLAVDKSMQGQGLGAQLLLSAGKRCIQAALQVGGVALLIDAKNKQVCDWYKGFGAVPLNDQPLSLLL
SFKTLYAALSASGRL

Nucleotide

• Length: 528 bp

>T185246 NZ_CP088120:c4694335-4694051 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10664.50 Da
• Isoelectric Point: 6.3133

>AT185246 WP_000855694.1 NZ_CP066851:3887407-3887697 [Salmonella enterica subsp. enterica serovar Heidelberg]
MKTMPQIAIESNERLSLRVSTDAKKLIVRAAAIQQTNLTDFVVSNVLPVAQKIVDAAERVYLTERDTQMIMEILDNPPAP
NEKLLAAAFALPDMKK

Nucleotide

• Length: 291 bp

>AT185246 NZ_CP088120:c4694567-4694325 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A633KU44

Antitoxin

• AlphaFold DB: A0A3V8SJE7