Detailed information of TA system TA185232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 840579..841155
• Replicon: chromosome
• Accession: NZ_CP066851
• Organism: Salmonella enterica subsp. enterica serovar Heidelberg strain SH-2813-Parental

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: JIG31_RS03920
• Protein ID: WP_001131963.1
• Coordinates: 840579..840866 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: JIG31_RS03925
• Protein ID: WP_000063142.1
• Coordinates: 840853..841155 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JIG31_RS03910 (835689)	835689..839198	-	3510	WP_001043484.1	type I restriction-modification system endonuclease	-
JIG31_RS03915 (839396)	839396..840310	+	915	WP_000290512.1	restriction endonuclease	-
JIG31_RS03920 (840579)	840579..840866	+	288	WP_001131963.1	BrnT family toxin	Toxin
JIG31_RS03925 (840853)	840853..841155	+	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
JIG31_RS03930 (841230)	841230..842186	-	957	WP_000187839.1	GTPase	-
JIG31_RS03935 (842197)	842197..842400	-	204	WP_000460616.1	YbdD/YjiX family protein	-
JIG31_RS03940 (842495)	842495..844645	-	2151	WP_000379927.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T185232 WP_001131963.1 NZ_CP066851:840579-840866 [Salmonella enterica subsp. enterica serovar Heidelberg]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T185232 NZ_CP088119:c1160518-1160300 [Enterobacter kobei]
ATGTCTGAAAAACCACTCACAAAGGTCGATTATTTGATGCGCTTACGTCGTTGTCAGTCAATTGACACCCTCGAACGTGT
GATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCTGACCATCGCCTCGCTGAGC
TAACGATGAATAAGTTATATGACAAAATCCCCACATCCGTGTGGAAATTTGTTCGTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT185232 WP_000063142.1 NZ_CP066851:840853-841155 [Salmonella enterica subsp. enterica serovar Heidelberg]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT185232 NZ_CP088119:c1160920-1160546 [Enterobacter kobei]
ATGGACGAGTACTCGCCAAAACGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGATCCAACGTCTGCCATCAATTTACAGTTGAACGAGCTTATCGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACGTTTATGTTGTTCAGTAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGCAAATCGGGAAACCGACT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCCGTTAGCCTTTCCTGTTAA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01