Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 103867..104510 Replicon plasmid pZ30-TEM-1B
Accession NZ_CP066845
Organism Escherichia coli strain Z30

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag JHL13_RS23300 Protein ID WP_001034044.1
Coordinates 104094..104510 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag JHL13_RS23295 Protein ID WP_001261286.1
Coordinates 103867..104097 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JHL13_RS23280 (99004) 99004..99234 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
JHL13_RS23285 (99231) 99231..99647 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
JHL13_RS23290 (99692) 99692..103486 - 3795 WP_001144731.1 hypothetical protein -
JHL13_RS23295 (103867) 103867..104097 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JHL13_RS23300 (104094) 104094..104510 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
JHL13_RS23305 (104585) 104585..106150 + 1566 WP_001128474.1 AAA family ATPase -
JHL13_RS23310 (106135) 106135..107157 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD vat / iutA / iucD / iucC / iucB / iucA 1..127534 127534
- flank IS/Tn - - 107411..107914 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T185228 WP_001034044.1 NZ_CP066845:104094-104510 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T185228 NZ_CP088119:c72220-71879 [Enterobacter kobei]
TTGAAAATACTACCTGCTACAACTTCGCGGGCGGCGAAGCCCTGCCTGTCACCCGTTGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGTCTTACCCTAAACGATACTCCTTTCAGTGATGAAACCGTTATTCAGAATCACATTA
ATGCCGGGATCACGCTGGCCGATGCTGTTAACTTTCTGGTGGAAAAATACGAACTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCCCCTTATCTTCGGGCTGTAGATATCCTGCGGGCAAGACAGGCAACTGGCCTTTTACGACAAAG
CCGTAACACCGCAGTACGGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT185228 WP_001261286.1 NZ_CP066845:103867-104097 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT185228 NZ_CP088119:c72558-72241 [Enterobacter kobei]
ATGAGCAACATCACTTGGGGCCTGCAGCGCAATATCACGCCGTGCCTGGGAGCCCGCCTTGTGCAGGAGGGGAACCGGCT
GCACTACCTGGCTGACCGGACCAGTATCACAGGTAAGTTCAGTGATGCCGAATGTTTTAAGCTGGATGTGGCATTTCCGC
ACTTTATCAGTCAGATGGAGTCGATGCTTACCACTGGTGAAATGAACCCTCACCATGCCCACTACGTAATCCTGTATCAC
AACGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTATGTATACGTCGCTGTTTATTCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References