Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 99004..99647 Replicon plasmid pZ30-TEM-1B
Accession NZ_CP066845
Organism Escherichia coli strain Z30

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag JHL13_RS23285 Protein ID WP_001034046.1
Coordinates 99231..99647 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag JHL13_RS23280 Protein ID WP_001261278.1
Coordinates 99004..99234 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JHL13_RS23250 (94514) 94514..94819 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
JHL13_RS23255 (94821) 94821..95039 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
JHL13_RS23260 (95607) 95607..96119 + 513 WP_000151784.1 hypothetical protein -
JHL13_RS23265 (96153) 96153..97286 - 1134 WP_000545984.1 DUF3800 domain-containing protein -
JHL13_RS23270 (97453) 97453..98226 - 774 WP_000905949.1 hypothetical protein -
JHL13_RS23275 (98239) 98239..98739 - 501 WP_000528932.1 HEPN family nuclease -
JHL13_RS23280 (99004) 99004..99234 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JHL13_RS23285 (99231) 99231..99647 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
JHL13_RS23290 (99692) 99692..103486 - 3795 WP_001144731.1 hypothetical protein -
JHL13_RS23295 (103867) 103867..104097 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
JHL13_RS23300 (104094) 104094..104510 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD vat / iutA / iucD / iucC / iucB / iucA 1..127534 127534


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T185227 WP_001034046.1 NZ_CP066845:99231-99647 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T185227 NZ_CP088118:150394-150780 [Sinorhizobium meliloti]
GTGATTGTCGACACCTCCGCGCTGGTCGCCATCCTTTACCGCGAGCCCCAAGCGGCAAGCTTTGTCAACTATATCCACGA
CGCCGAAACCACTCGGATCAGCGTCGCTAACTATGTCGAGTTGTCCATGGTGGTGGAAAGCCAGCTCGGACCTGACGGTA
TGCGCCAGGCCGAAGCCTTCTTCCGGCGGGCCGGCATCATTGTTGAGCCGGTCACGCTTGAACATGGCGAACTGGCGCGT
CAGGCTTTTCTCGACTTCGGCAAGGGCCGGCACAAGGCCGGTCTCAATTTCGGCGATTGCTTCGCCTACGCCCTCGCTAA
AGCGTCTGGCGAACCGCTACTGTTCAAGGGCAATGACTTCTCTCAAACTGATGTCCTGACCGTCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT185227 WP_001261278.1 NZ_CP066845:99004-99234 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT185227 NZ_CP088118:150149-150397 [Sinorhizobium meliloti]
GTGAGCCTCAGCATCAAGGATCCAGAAGCCCATCGTCTGGCGCAGGCGATTTCCCACGCGACGGGGGAAAGCATGACCCG
CGTCGTCACCAATGCACTGCGCGAGCGCCTTGCCAAGATCGAACGCCAAAAGGGCCGCGCCAGCGTTGAGGAACTGCTGG
CGATCGCCGACCGTGCCGCCACGCACGTCAAACACCCTTATGACGATCATGCCGACTTTCTTTATGACGAGAACGGCATT
TCTAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References