Detailed information of TA system TA185222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 49046..49671
• Replicon: plasmid pZ30-TEM-1B
• Accession: NZ_CP066845
• Organism: Escherichia coli strain Z30

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JHL13_RS22960
• Protein ID: WP_000911313.1
• Coordinates: 49273..49671 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: JHL13_RS22955
• Protein ID: WP_000450520.1
• Coordinates: 49046..49273 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHL13_RS22955 (49046)	49046..49273	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JHL13_RS22960 (49273)	49273..49671	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
JHL13_RS22965 (49680)	49680..51833	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
JHL13_RS22970 (52086)	52086..52817	-	732	WP_023565183.1	conjugal transfer complement resistance protein TraT	-
JHL13_RS22975 (52849)	52849..53346	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	vat / iutA / iucD / iucC / iucB / iucA	1..127534	127534

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T185222 WP_000911313.1 NZ_CP066845:49273-49671 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T185222 NZ_CP088115:c300589-300335 [Sinorhizobium meliloti]
ATGAACAGCAAGCAGAGAAAGACCCTCGCGGCCGTATTCAAGGACCCGGCCTCGGGAACCATTGAATGGGCCGCTATAGA
GAATCTGCTTGTGGCTGCCGGAGCGGACGTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAAGACGGCATCATTG
CGAGCTTTCATCGACCTCATCCGGACAAGGAAGCAAAGCGTTATCAGGTGCGCGATGCTCGCGAGTTCCTGATGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT185222 WP_000450520.1 NZ_CP066845:49046-49273 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT185222 NZ_CP088115:c300338-299982 [Sinorhizobium meliloti]
ATGAACACCATGACCTACAACGGGTATCACGCTCGCATCGAATTCGACGCTGAGGACGAAGTGTTTTTCGGCAAGATTGC
AGGTATCTCCGATGTAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCGGTCGATGACT
ACCTCGAGACCTGCAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTCAACCAGTGGGCAGAAGAGGTGCTGGAAAAAGC
CGCCGGCCATTTTGCCGCGGCGCGCCCGTCGGCGTAG

Structures

Antitoxin

• AlphaFold DB: V0VCB2