Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4243064..4243561 Replicon chromosome
Accession NZ_CP066844
Organism Escherichia coli strain Z30

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JHL13_RS20025 Protein ID WP_001173343.1
Coordinates 4243064..4243237 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JHL13_RS20030 Protein ID WP_000398619.1
Coordinates 4243265..4243561 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JHL13_RS20015 (4239734) 4239734..4241191 + 1458 WP_226153419.1 dipeptide/tripeptide permease DtpC -
JHL13_RS20020 (4241428) 4241428..4242945 + 1518 WP_000003800.1 lysine--tRNA ligase -
JHL13_RS20025 (4243064) 4243064..4243237 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JHL13_RS20030 (4243265) 4243265..4243561 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JHL13_RS20035 (4243790) 4243790..4244062 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
JHL13_RS20040 (4244074) 4244074..4244304 - 231 WP_001683478.1 4Fe-4S mono-cluster protein YjdI -
JHL13_RS20045 (4244485) 4244485..4246116 + 1632 WP_022646455.1 sensor histidine kinase -
JHL13_RS20050 (4246113) 4246113..4246832 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4234210..4243561 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T185219 WP_001173343.1 NZ_CP066844:c4243237-4243064 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T185219 NZ_CP088114:c962201-961647 [Sinorhizobium meliloti]
ATGAGGAAACACATTCCGCCCGCATCGCGACCCGATCCGGAACCGACGTCGCCGGTCGAGCCGATAACGCTCTGGCGCGC
CTTCGTTCCGCGCTGGGCACATATGCCGCTATCCGGCGCGGGTGCCGCTCGCTTCGGTGGCCGCTGGAACCCGATCGGCA
TGCCTGCGATTTATGCTGCGCGCGAGCTTTCCACTGCCTGGGCTGAATATAATCAGGGTTTCGTGCAGCATCCGGCGCTC
ATCGTGCAGCTCGAGTTGCGCGGGGCGAGGCTCGCGGACCTCACGGATGCCAGCGTTCTTCTGGAGCTTGGTGTGGATGA
GGCGATCCATCGCTGCGAATGGCGTGATGCGCTCGACAAAGGCGCGGTGCCGGAGACCCACAGGCCGCAGTCGGAGCTTC
TGGCGCGCGACTATCACGGGGTGATCTATCCATCCTTCATGTCGCCCGGCGGCACCTGCGTCGCGCTCTGGCGCTGGAAC
GGTGCGGAAGAACCGCGTCTCGACGTCATCGATCCCGATGGCCGGCTGCCGAAATCGCCCGCCTCATGGGTTTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT185219 WP_000398619.1 NZ_CP066844:c4243561-4243265 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT185219 NZ_CP088114:c962579-962208 [Sinorhizobium meliloti]
ATGATGGCCGTTGATTTCCAGATACCTGCAGCCCGTTTCGGGGACGATCATTCCCCGTTTCTCTCGGCCCGCCTTGTCGC
CGACCGGTTGGGCGTCACGCTCGCCGAACTCGCCAAGCTGATCGGTGTCGCCCGCAATACGCTGACGGCGAAATCCGGCG
CCCGGAAGGTCGACAGCGCCTTGAGCAAGGTCGTCCGCATCCTCGCCATGGCTTCCGAAATGGCGGGCGACGAAGCCCGC
GCCGTCATCTGGTTCAAGCACCAGCCTATCCCCGGCTGGGCCGGCAAGACGGCGTTCGATCTGGTCGGCGAGGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAATCGGTACGTGCCGGCGTCTACGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References