Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3907419..3907969 Replicon chromosome
Accession NZ_CP066844
Organism Escherichia coli strain Z30

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag JHL13_RS18510 Protein ID WP_001160966.1
Coordinates 3907419..3907733 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag JHL13_RS18515 Protein ID WP_000125568.1
Coordinates 3907736..3907969 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JHL13_RS18485 (3903094) 3903094..3904380 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
JHL13_RS18490 (3904380) 3904380..3905369 + 990 WP_000241224.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
JHL13_RS18495 (3905366) 3905366..3906187 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
JHL13_RS18500 (3906190) 3906190..3906567 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
JHL13_RS18505 (3906574) 3906574..3907422 + 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
JHL13_RS18510 (3907419) 3907419..3907733 - 315 WP_001160966.1 CcdB family protein Toxin
JHL13_RS18515 (3907736) 3907736..3907969 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
JHL13_RS18520 (3908055) 3908055..3908534 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
JHL13_RS18525 (3908726) 3908726..3910588 - 1863 WP_022645165.1 glutathione-regulated potassium-efflux system protein KefC -
JHL13_RS18530 (3910581) 3910581..3911111 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
JHL13_RS18535 (3911219) 3911219..3912550 - 1332 WP_001183201.1 MFS transporter -
JHL13_RS18540 (3912608) 3912608..3912895 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T185217 WP_001160966.1 NZ_CP066844:c3907733-3907419 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T185217 NZ_CP088113:c2605034-2604636 [Sinorhizobium meliloti]
ATGCTCACCTATATGCTCGATACCAATATCTGCATCTATGTGATGAAGACCTATCCGCCGGCCGTGCGCGAAAAGTTCAA
CGGACTGGCGGAGCAGCTTTGCATATCGAGCATCACGCTGGGAGAATTACACTATGGCGCTGAAAAATCGGCCCGGCGCG
TTGAGAACCTTACGGCTATCGAACATTTCGTGGCACGGCTGGAGGTGCTGCCGTTCGCAGACAAGGCAGCGGCCCACTAC
GGGCAGGTTCGGGCTGAACTGGAGCGGACGGGAACGCCATGCGGCCCTCATGACATGCAGATCGGCGCGCATGCGCGCAG
CGAAGGGCTGATTGTCGTGACCAACAATATACGCGAGTTCGTCCGCATGCCGGGTGTAAGGGTCGAGAACTGGCTGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT185217 WP_000125568.1 NZ_CP066844:c3907969-3907736 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT185217 NZ_CP088113:c2605267-2605034 [Sinorhizobium meliloti]
TTGGCCAGTTCGACCGTATTCATCAGTAACCGTAGCCAGGCGGTGCGGCTGCCAAAGGCCGTCGCCTTTCCCGAGGGCGT
GCATCAGGTCGATATCCTCAAGATCGGCCGCAGCCGCGTGATCGTCCCCCAGGGCAAAAGATGGGACGATCTGTTCCTCA
GCGGGCCGCGCGTCAGCGAGGATTTCATGAGCGAGCGTGATCAGCCGGTGGCGGAAACACGCGAATCCTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References