Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3487400..3488018 Replicon chromosome
Accession NZ_CP066844
Organism Escherichia coli strain Z30

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JHL13_RS16535 Protein ID WP_001291435.1
Coordinates 3487800..3488018 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JHL13_RS16530 Protein ID WP_000344800.1
Coordinates 3487400..3487774 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JHL13_RS16520 (3482489) 3482489..3483682 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JHL13_RS16525 (3483705) 3483705..3486854 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JHL13_RS16530 (3487400) 3487400..3487774 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JHL13_RS16535 (3487800) 3487800..3488018 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JHL13_RS16540 (3488190) 3488190..3488741 + 552 WP_000536391.1 maltose O-acetyltransferase -
JHL13_RS16545 (3488857) 3488857..3489327 + 471 WP_022645280.1 YlaC family protein -
JHL13_RS16550 (3489491) 3489491..3491041 + 1551 WP_012602175.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JHL13_RS16555 (3491082) 3491082..3491435 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JHL13_RS16565 (3491814) 3491814..3492125 + 312 WP_000409911.1 MGMT family protein -
JHL13_RS16570 (3492156) 3492156..3492728 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T185215 WP_001291435.1 NZ_CP066844:3487800-3488018 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T185215 NZ_CP088113:2275963-2276367 [Sinorhizobium meliloti]
TTGAACGGGTATCTTCTCGATACGAACATCATCAGCGACGTCATTCACAATCCGTTCGGACCGGCTGCGCAACGCATCGA
ACGGATCGGCCCGAAAGAGATTTACACCAGCATCGTCGTCGCGTCCGAGTTGCGGTATGGTTGCGCAAAAAAGGGATCGG
CGAAGTTGCTCGCCAAGGTCGAAAGCTTGCTGGAAATCGTCCCGGTGCTGCCATTGGATATTCCGGCCGACACCCGATAT
GGCAGCATTCGTGCGGAATTGGAGTCTCTGGGGCAGACCATCGGCTCCAACGATCTGTTGATTGCCGCGCATGCGTATGC
GCTGGACCTGACGCTGGTGACGGACAATATCCGTGAGTTCAGCCGCGTTCGTGGCCTTAGTCTGGAAAACTGGCTTGAGC
GATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT185215 WP_000344800.1 NZ_CP066844:3487400-3487774 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT185215 NZ_CP088113:2275694-2275966 [Sinorhizobium meliloti]
ATGCCCGTCCCGTTGCCGTCATCGCGACCTAAAGAAGTAAAGCTGTTCCGCAATAACCGCAGCCAGGCAGTCCGTATTCC
TGCGGAGTTCGAGTTGCCTGGAGACCGTGTGTTGATCCGTCGCGAAGGAACGAGACTCATTATCGAACCGATAGCCAGGC
CAGCCGATATCGTGGAGCTTCTCGCGGAGTGGAAGAAGGAAGCTCCCCTCGGACCTGAAGACCGGTTCCCGGATGTCGAG
GATATACCCGCCCGGCCGGAGAAAATCTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References