Detailed information of TA system TA185213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3073997..3074702
• Replicon: chromosome
• Accession: NZ_CP066844
• Organism: Escherichia coli strain Z30

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: JHL13_RS14700
• Protein ID: WP_000539521.1
• Coordinates: 3073997..3074383 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JHL13_RS14705
• Protein ID: WP_001280945.1
• Coordinates: 3074373..3074702 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHL13_RS14680 (3070001)	3070001..3070627	+	627	WP_001295292.1	glutathione S-transferase GstB	-
JHL13_RS14685 (3070624)	3070624..3071739	-	1116	WP_000554964.1	aldose sugar dehydrogenase YliI	-
JHL13_RS14690 (3071850)	3071850..3072233	-	384	WP_000497137.1	biofilm formation regulator BssR	-
JHL13_RS14695 (3072446)	3072446..3073771	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JHL13_RS14700 (3073997)	3073997..3074383	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JHL13_RS14705 (3074373)	3074373..3074702	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JHL13_RS14710 (3074772)	3074772..3076100	-	1329	WP_022645414.1	GGDEF domain-containing protein	-
JHL13_RS14715 (3076108)	3076108..3078456	-	2349	WP_022645413.1	EAL domain-containing protein	-
JHL13_RS14720 (3078633)	3078633..3079544	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T185213 WP_000539521.1 NZ_CP066844:3073997-3074383 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T185213 NZ_CP088113:c1209984-1209697 [Sinorhizobium meliloti]
ATGCCCTATAGCCTCGAATTCCTGCCGTCTGCTCGAAAGGAATGGGACAAGCTGGGCGCGACGATCCGGCAGCAGTTTGT
AAAGAAGCTGCGTGAGCGTTTGGAGCGCCCCCGCATTCCGAGCGCCGCACTACACGGGATGCCCGACCACTACAAAATCA
AACTGAGACAGCTGGGCTATCGTCTCGTCTACCGGGTCGATGACGGCGTTGTCACTGTGCTGGCCGTGGCAGTTGGAAAG
CGAGAGCGGGGTGACGTCTACAACTTGATGCGAGCACGCGGTCGTTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT185213 WP_001280945.1 NZ_CP066844:3074373-3074702 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT185213 NZ_CP088113:c1210219-1209974 [Sinorhizobium meliloti]
ATGCAGCGTGTAGAAGCCTCTGTGGCCGTGAGCGTTTCCGACTTGAAGAAGAGTCCGACGGCAGTAATGGGCGAGGCTCA
AGGCGAGCCCGTGGCCGTGCTGAACCACAATCGTGTCATGGCCTATCTGGTGCCTGCCGATGTCTACGAATCCATGCTTG
AGCGGCTGGACGACCTCTATCTTGCCGAGCTTGTGAAAGAGCGGGCGGACGAGAAGGGCGAGCCGGTCGATATTGATGCC
CTATAG

Structures

Toxin

• AlphaFold DB: A0A829GCT6