Detailed information of TA system TA185212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2819385..2820169
• Replicon: chromosome
• Accession: NZ_CP066844
• Organism: Escherichia coli strain Z30

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: JHL13_RS13500
• Protein ID: WP_000613626.1
• Coordinates: 2819675..2820169 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: JHL13_RS13495
• Protein ID: WP_139352217.1
• Coordinates: 2819385..2819678 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHL13_RS13485 (2814534)	2814534..2815493	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
JHL13_RS13490 (2816066)	2816066..2819251	+	3186	WP_077516769.1	ribonuclease E	-
JHL13_RS13495 (2819385)	2819385..2819678	+	294	WP_139352217.1	DUF1778 domain-containing protein	Antitoxin
JHL13_RS13500 (2819675)	2819675..2820169	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
JHL13_RS13505 (2820264)	2820264..2821217	-	954	WP_001212763.1	flagellar hook-associated protein FlgL	-
JHL13_RS13510 (2821229)	2821229..2822872	-	1644	WP_077516765.1	flagellar hook-associated protein FlgK	-
JHL13_RS13515 (2822938)	2822938..2823879	-	942	WP_012311956.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
JHL13_RS13520 (2823879)	2823879..2824976	-	1098	WP_000589300.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-95)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T185212 WP_000613626.1 NZ_CP066844:2819675-2820169 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T185212 NZ_CP088113:c455906-455520 [Sinorhizobium meliloti]
ATGGTCATCGACACATCCGCCATTGCCGCGATTGCCTTCAATGAACAGGAGGCCGGAAGTTTCCGCGAAAAAATCGCGGA
CGACCCGGTTCGGCTCATCTCCGCGGCAACCGCTCTGGAAGCAGCCATGGTAATCGAAACCCGGTTAGGCGAAGCTGCCG
GAGCGGAACTTGATCTTTGGCTTTACAAGGCAAACGTGGAGATTGTTGCGGTAACCGCCGAGCATATGGACCAGGCCCGC
CGTGCCTGGCGCCGTTTCGGCAAAGGCCGTCATCCGGCCGGTTTGAATTTCGGTGATTGTTTTTCCTATGCCCTGGCCTT
TCTGACAAATGAACCGCTTCTCTTCAAGGGAAGCGATTTCTCGCAAACCGATATCCCGGCGGCGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11139.76 Da
• Isoelectric Point: 10.5423

>AT185212 WP_139352217.1 NZ_CP066844:2819385-2819678 [Escherichia coli]
MPAANNIPARREFLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT185212 NZ_CP088113:c456160-455906 [Sinorhizobium meliloti]
ATGGCGCTGAGCATCAAGGACACTGAAACCGAGCAGCTTGCCCGTACACTTGCCCGGCGGACAGGGGAATCCATCACGCT
TGCCACCAAGCGTGCCCTCGAAGAACGATTGCGCCGAACCGACACCGCTGCCCGGCGCGCGGCTCTTCTGGAGGATCTGG
AGGCCATTCAACGCCGCTGGAATGCCATGCCCGTCCTCGACAGTCGCAGCTCCGATGACATTATCGGTTATGACGAGGAT
GGACTCCCCTCATAA

Structures

Toxin

• AlphaFold DB: V0T0H9