Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2466859..2467497 Replicon chromosome
Accession NZ_CP066844
Organism Escherichia coli strain Z30

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JHL13_RS11810 Protein ID WP_000813794.1
Coordinates 2467321..2467497 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JHL13_RS11805 Protein ID WP_001270286.1
Coordinates 2466859..2467275 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JHL13_RS11785 (2462011) 2462011..2462952 - 942 WP_088129997.1 ABC transporter permease -
JHL13_RS11790 (2462953) 2462953..2463966 - 1014 WP_022645666.1 ABC transporter ATP-binding protein -
JHL13_RS11795 (2463984) 2463984..2465129 - 1146 WP_000047466.1 ABC transporter substrate-binding protein -
JHL13_RS11800 (2465374) 2465374..2466780 - 1407 WP_022645665.1 PLP-dependent aminotransferase family protein -
JHL13_RS11805 (2466859) 2466859..2467275 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JHL13_RS11810 (2467321) 2467321..2467497 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JHL13_RS11815 (2467719) 2467719..2467949 + 231 WP_000491567.1 DUF2554 family protein -
JHL13_RS11820 (2468041) 2468041..2470002 - 1962 WP_023909195.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JHL13_RS11825 (2470075) 2470075..2470611 - 537 WP_000429148.1 DNA-binding transcriptional regulator SutR -
JHL13_RS11830 (2470703) 2470703..2471875 + 1173 WP_022645663.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T185211 WP_000813794.1 NZ_CP066844:c2467497-2467321 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T185211 NZ_CP088113:239124-239387 [Sinorhizobium meliloti]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCCTCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT185211 WP_001270286.1 NZ_CP066844:c2467275-2466859 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT185211 NZ_CP088113:239384-239701 [Sinorhizobium meliloti]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCAAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References